Conjugated linoleic acid inhibits cell proliferation, independent of NF-κB activation in D5H3 cells

W. Wang, S. V. Gupta, T. V. Fungwe, R. Sen, P. Khosla

Research output: Contribution to journalArticlepeer-review

Abstract

Conjugated linoleic acid (CLA), a mixture of positional & geometrical isomere of linoleic acid (LA), occurs naturally in meat & dairy products. In vivo and in vitro studies have shown CLA to be an effective anticarcinogen, although the mechanism is unclear. Since inhibition of NF-κB (a ubiquitous nuclear transcription factor) has been implicated in cell death, the role of CLA in affecting NF-kB induction was evaluated. T cell hybridoma D5h3 cells were grown for 3 generations in a) Control media - CM- (DMEM with 10% IFS, 2 mM glutamine, 10 mM HEPES) or b) CM + BSA-bound CLA (15μM, 30μM & 60μM) or c) CM + BSA-bound LA (15μM, 30μM & 60μM). Cells were then incubated with PMA (50 ng/ml) + ionomycin (2μM) or anti-CD3 for 4 hr. Nuclear extracts were prepared and EMSA performed using a labeled H2K fragment containing an NF-κB consensus site. Induction of NF-κB binding affinity was similar in cells grown in CLA or LA and higher than those grown in CM. Cell viability was not affected by CLA or LA. However, increasing concentrations of CLA significantly suppressed cell proliferation (by 50%, 80% & 90%, respectively), whereas LA had no effect, suggesting that CLA-induced reduction in cell number was due to an effect on cell proliferation rather than cell death. Studies testing the former possibility, are in progress.

Original languageEnglish (US)
JournalFASEB Journal
Volume12
Issue number5
StatePublished - Mar 20 1998
Externally publishedYes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

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