Conjugate systems using delocalized cationic dyes as a carrier of photosensitizers to mitochondria

Youngjae You, Ethel J. Ngen, Pallavi Rajaputra

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Following Photofrin, the first generation photosensitizer, several second generation photosensitizers have been developed with improved characteristics. More recently, third generation photosensitizers are proposed to achieve higher selectivity toward cancer cells/ tumor tissue. Elevated mitochondrial membrane potential of malignant cells has tested as a tool for preferential uptake of certain photosensitizers to cancer cells. In the same line, we designed new conjugate systems where delocalized cationic moiety delivers a photosensitizer to mitochondria. To prove our concept, two prototype conjugates (TPP-Rh and TPP-AO) were prepared using two cationic dyes (Rhodamine and Acridine Orange) and a photosensitizer (tetraphenylporphyrin, TPP). The two conjugates generated singlet oxygen quite well. Interestingly, the two conjugates showed higher cellular uptake by mover than 8 times than TPP-OH as well as higher phototoxicity. In particular, TPP-Rh showed closer localization pattern to mitochondria than TPP-OH.

Original languageEnglish (US)
Title of host publication12th World Congress of the International Photodynamic Association - Photodynamic Therapy
Subtitle of host publicationBack to the Future
DOIs
StatePublished - Dec 1 2009
Externally publishedYes
Event12th World Congress of the International Photodynamic Association - Photodynamic Therapy: Back to the Future - Seattle, WA, United States
Duration: Jun 11 2009Jun 15 2009

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7380
ISSN (Print)1605-7422

Other

Other12th World Congress of the International Photodynamic Association - Photodynamic Therapy: Back to the Future
CountryUnited States
CitySeattle, WA
Period6/11/096/15/09

Keywords

  • Conjugate
  • acridine orange
  • mitochondria
  • rhodamine

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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