Congenital Trypanosoma cruzi Transmission in Santa Cruz, Bolivia

Caryn Bern, Manuela Verastegui, Robert H Gilman, Carlos LaFuente, Gerson Galdos-Cardenas, Maritza Calderon, Juan Pacori, Maria Del Carmen Abastoflor, Hugo Aparicio, Mark F. Brady, Lisbeth Ferrufino, Noelia Angulo, Sarah Marcus, Charles Sterling, James H. Maguire

Research output: Contribution to journalArticle

Abstract

Background: We conducted a study of congenital Trypanosoma cruzi infection in Santa Cruz, Bolivia. Our objective was to apply new tools to identify weak points in current screening algorithms, and find ways to improve them. Methods: Women presenting for delivery were screened by rapid and conventional serological tests. For infants of infected mothers, blood specimens obtained on days 0, 7, 21, 30, 90, 180, and 270 were concentrated and examined microscopically; serological tests were performed for the day 90, 180, and 270 specimens. Maternal and infant specimens, including umbilical tissue, were tested by polymerase chain reaction (PCR) targeting the kinetoplast minicircle and by quantitative PCR. Results: Of 530 women, 154 (29%) were seropositive. Ten infants had congenital T. cruzi infection. Only 4 infants had positive results of microscopy evaluation in the first month, and none had positive cord blood microscopy results. PCR results were positive for 6 (67%) of 9 cord blood and 7 (87.5%) of 8 umbilical tissue specimens. PCR-positive women were more likely to transmit T. cruzi than were seropositive women with negative PCR results (P

Original languageEnglish (US)
Pages (from-to)1667-1674
Number of pages8
JournalClinical Infectious Diseases
Volume49
Issue number11
DOIs
StatePublished - Dec 2009

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Bolivia
Trypanosoma cruzi
Polymerase Chain Reaction
Umbilicus
Serologic Tests
Fetal Blood
Microscopy
Mothers
Infection

ASJC Scopus subject areas

  • Infectious Diseases
  • Microbiology (medical)

Cite this

Bern, C., Verastegui, M., Gilman, R. H., LaFuente, C., Galdos-Cardenas, G., Calderon, M., ... Maguire, J. H. (2009). Congenital Trypanosoma cruzi Transmission in Santa Cruz, Bolivia. Clinical Infectious Diseases, 49(11), 1667-1674. https://doi.org/10.1086/648070

Congenital Trypanosoma cruzi Transmission in Santa Cruz, Bolivia. / Bern, Caryn; Verastegui, Manuela; Gilman, Robert H; LaFuente, Carlos; Galdos-Cardenas, Gerson; Calderon, Maritza; Pacori, Juan; Del Carmen Abastoflor, Maria; Aparicio, Hugo; Brady, Mark F.; Ferrufino, Lisbeth; Angulo, Noelia; Marcus, Sarah; Sterling, Charles; Maguire, James H.

In: Clinical Infectious Diseases, Vol. 49, No. 11, 12.2009, p. 1667-1674.

Research output: Contribution to journalArticle

Bern, C, Verastegui, M, Gilman, RH, LaFuente, C, Galdos-Cardenas, G, Calderon, M, Pacori, J, Del Carmen Abastoflor, M, Aparicio, H, Brady, MF, Ferrufino, L, Angulo, N, Marcus, S, Sterling, C & Maguire, JH 2009, 'Congenital Trypanosoma cruzi Transmission in Santa Cruz, Bolivia', Clinical Infectious Diseases, vol. 49, no. 11, pp. 1667-1674. https://doi.org/10.1086/648070
Bern C, Verastegui M, Gilman RH, LaFuente C, Galdos-Cardenas G, Calderon M et al. Congenital Trypanosoma cruzi Transmission in Santa Cruz, Bolivia. Clinical Infectious Diseases. 2009 Dec;49(11):1667-1674. https://doi.org/10.1086/648070
Bern, Caryn ; Verastegui, Manuela ; Gilman, Robert H ; LaFuente, Carlos ; Galdos-Cardenas, Gerson ; Calderon, Maritza ; Pacori, Juan ; Del Carmen Abastoflor, Maria ; Aparicio, Hugo ; Brady, Mark F. ; Ferrufino, Lisbeth ; Angulo, Noelia ; Marcus, Sarah ; Sterling, Charles ; Maguire, James H. / Congenital Trypanosoma cruzi Transmission in Santa Cruz, Bolivia. In: Clinical Infectious Diseases. 2009 ; Vol. 49, No. 11. pp. 1667-1674.
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