Confirmatory analysis of buprenorphine, norbuprenorphine, and glucuronide metabolites in plasma by LCMSMS. Application to umbilical cord plasma from buprenorphine-maintained pregnant women

Marta Concheiro, Hendreé Jones, Rolley E. Johnson, Diaa M. Shakleya, Marilyn A. Huestis

Research output: Contribution to journalArticle

Abstract

An LCMSMS method was developed and fully validated for the simultaneous quantification of buprenorphine (BUP), norbuprenorphine (NBUP), buprenorphine-glucuronide (BUP-Gluc), and norbuprenorphine-glucuronide (NBUP-Gluc) in 0.5 mL plasma, fulfilling confirmation criteria with two transitions for each compound with acceptable relative ion intensities. Transitions monitored were 468.3 > 396.2 and 468.3 > 414.3 for BUP, 414.3 > 340.1 and 414.3 > 326.0 for NBUP, 644.3 > 468.1 and 644.3 > 396.3 for BUP-Gluc, and 590.3 > 414.3 and 590.3 > 396.2 for NBUP-Gluc. Linearity was 0.1-50 ng/mL for BUP and BUP-Gluc, and 0.5-50 ng/mL for NBUP and NBUP-Gluc. Intra-day, inter-day, and total assay imprecision (%RSD) were

Original languageEnglish (US)
Pages (from-to)13-20
Number of pages8
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume878
Issue number1
DOIs
StatePublished - Jan 1 2009

Fingerprint

Buprenorphine
Umbilical Cord
Glucuronides
Metabolites
Pregnant Women
Plasmas
norbuprenorphine
Assays
Ions

Keywords

  • Buprenorphine
  • LCMSMS
  • Plasma
  • Umbilical cord plasma

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Cell Biology
  • Clinical Biochemistry

Cite this

Confirmatory analysis of buprenorphine, norbuprenorphine, and glucuronide metabolites in plasma by LCMSMS. Application to umbilical cord plasma from buprenorphine-maintained pregnant women. / Concheiro, Marta; Jones, Hendreé; Johnson, Rolley E.; Shakleya, Diaa M.; Huestis, Marilyn A.

In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 878, No. 1, 01.01.2009, p. 13-20.

Research output: Contribution to journalArticle

@article{7a736d0447b04ed89012f11042d75647,
title = "Confirmatory analysis of buprenorphine, norbuprenorphine, and glucuronide metabolites in plasma by LCMSMS. Application to umbilical cord plasma from buprenorphine-maintained pregnant women",
abstract = "An LCMSMS method was developed and fully validated for the simultaneous quantification of buprenorphine (BUP), norbuprenorphine (NBUP), buprenorphine-glucuronide (BUP-Gluc), and norbuprenorphine-glucuronide (NBUP-Gluc) in 0.5 mL plasma, fulfilling confirmation criteria with two transitions for each compound with acceptable relative ion intensities. Transitions monitored were 468.3 > 396.2 and 468.3 > 414.3 for BUP, 414.3 > 340.1 and 414.3 > 326.0 for NBUP, 644.3 > 468.1 and 644.3 > 396.3 for BUP-Gluc, and 590.3 > 414.3 and 590.3 > 396.2 for NBUP-Gluc. Linearity was 0.1-50 ng/mL for BUP and BUP-Gluc, and 0.5-50 ng/mL for NBUP and NBUP-Gluc. Intra-day, inter-day, and total assay imprecision ({\%}RSD) were",
keywords = "Buprenorphine, LCMSMS, Plasma, Umbilical cord plasma",
author = "Marta Concheiro and Hendre{\'e} Jones and Johnson, {Rolley E.} and Shakleya, {Diaa M.} and Huestis, {Marilyn A.}",
year = "2009",
month = "1",
day = "1",
doi = "10.1016/j.jchromb.2009.11.005",
language = "English (US)",
volume = "878",
pages = "13--20",
journal = "Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences",
issn = "1570-0232",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Confirmatory analysis of buprenorphine, norbuprenorphine, and glucuronide metabolites in plasma by LCMSMS. Application to umbilical cord plasma from buprenorphine-maintained pregnant women

AU - Concheiro, Marta

AU - Jones, Hendreé

AU - Johnson, Rolley E.

AU - Shakleya, Diaa M.

AU - Huestis, Marilyn A.

PY - 2009/1/1

Y1 - 2009/1/1

N2 - An LCMSMS method was developed and fully validated for the simultaneous quantification of buprenorphine (BUP), norbuprenorphine (NBUP), buprenorphine-glucuronide (BUP-Gluc), and norbuprenorphine-glucuronide (NBUP-Gluc) in 0.5 mL plasma, fulfilling confirmation criteria with two transitions for each compound with acceptable relative ion intensities. Transitions monitored were 468.3 > 396.2 and 468.3 > 414.3 for BUP, 414.3 > 340.1 and 414.3 > 326.0 for NBUP, 644.3 > 468.1 and 644.3 > 396.3 for BUP-Gluc, and 590.3 > 414.3 and 590.3 > 396.2 for NBUP-Gluc. Linearity was 0.1-50 ng/mL for BUP and BUP-Gluc, and 0.5-50 ng/mL for NBUP and NBUP-Gluc. Intra-day, inter-day, and total assay imprecision (%RSD) were

AB - An LCMSMS method was developed and fully validated for the simultaneous quantification of buprenorphine (BUP), norbuprenorphine (NBUP), buprenorphine-glucuronide (BUP-Gluc), and norbuprenorphine-glucuronide (NBUP-Gluc) in 0.5 mL plasma, fulfilling confirmation criteria with two transitions for each compound with acceptable relative ion intensities. Transitions monitored were 468.3 > 396.2 and 468.3 > 414.3 for BUP, 414.3 > 340.1 and 414.3 > 326.0 for NBUP, 644.3 > 468.1 and 644.3 > 396.3 for BUP-Gluc, and 590.3 > 414.3 and 590.3 > 396.2 for NBUP-Gluc. Linearity was 0.1-50 ng/mL for BUP and BUP-Gluc, and 0.5-50 ng/mL for NBUP and NBUP-Gluc. Intra-day, inter-day, and total assay imprecision (%RSD) were

KW - Buprenorphine

KW - LCMSMS

KW - Plasma

KW - Umbilical cord plasma

UR - http://www.scopus.com/inward/record.url?scp=71449117665&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=71449117665&partnerID=8YFLogxK

U2 - 10.1016/j.jchromb.2009.11.005

DO - 10.1016/j.jchromb.2009.11.005

M3 - Article

C2 - 19945361

AN - SCOPUS:71449117665

VL - 878

SP - 13

EP - 20

JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

SN - 1570-0232

IS - 1

ER -