Confirmation of an ultrasound-induced mutation in two in-vitro mammalian cell lines

Yukio Doida; Morton W. Miller; Christopher Cox; Charles C. Church

doi:10.1016/0301-5629(90)90103-J

Confirmation of an ultrasound-induced mutation in two in-vitro mammalian cell lines

Yukio Doida, Morton W. Miller, Christopher Cox, Charles C. Church

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

In-vitro V79 and L5178Y cells were exposed in a rotating test tube to continuous-wave (CW) 1 MHz 35 W/cm² ultrasound (0-4 or 0-3 min, respectively) and subsequently assayed for mutation as evidenced by resistance to 6-thioguanine (6-TG). There was a modest but statistically significant increase in mutation frequency in both cell types with increase in ultrasound exposure duration. X-ray exposures (3-9 Gy, a "positive control") yielded a large increase in 6-TG resistance. The data support an earlier report by Kaufman (1985) of an ultrasound-induced increase in mutation to 6-TG resistance in in-vitro mammalian cells.

Original language	English (US)
Pages (from-to)	699-705
Number of pages	7
Journal	Ultrasound in Medicine and Biology
Volume	16
Issue number	7
DOIs	https://doi.org/10.1016/0301-5629(90)90103-J
State	Published - 1990
Externally published	Yes

Keywords

Acoustics
Cell mutation
In-vitro mammalian cells
Ultrasound
X-rays

ASJC Scopus subject areas

Radiological and Ultrasound Technology
Biophysics
Acoustics and Ultrasonics

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10.1016/0301-5629(90)90103-J

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title = "Confirmation of an ultrasound-induced mutation in two in-vitro mammalian cell lines",

abstract = "In-vitro V79 and L5178Y cells were exposed in a rotating test tube to continuous-wave (CW) 1 MHz 35 W/cm2 ultrasound (0-4 or 0-3 min, respectively) and subsequently assayed for mutation as evidenced by resistance to 6-thioguanine (6-TG). There was a modest but statistically significant increase in mutation frequency in both cell types with increase in ultrasound exposure duration. X-ray exposures (3-9 Gy, a {"}positive control{"}) yielded a large increase in 6-TG resistance. The data support an earlier report by Kaufman (1985) of an ultrasound-induced increase in mutation to 6-TG resistance in in-vitro mammalian cells.",

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doi = "10.1016/0301-5629(90)90103-J",

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journal = "Ultrasound in Medicine and Biology",

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T1 - Confirmation of an ultrasound-induced mutation in two in-vitro mammalian cell lines

AU - Doida, Yukio

AU - Miller, Morton W.

AU - Cox, Christopher

AU - Church, Charles C.

PY - 1990

Y1 - 1990

N2 - In-vitro V79 and L5178Y cells were exposed in a rotating test tube to continuous-wave (CW) 1 MHz 35 W/cm2 ultrasound (0-4 or 0-3 min, respectively) and subsequently assayed for mutation as evidenced by resistance to 6-thioguanine (6-TG). There was a modest but statistically significant increase in mutation frequency in both cell types with increase in ultrasound exposure duration. X-ray exposures (3-9 Gy, a "positive control") yielded a large increase in 6-TG resistance. The data support an earlier report by Kaufman (1985) of an ultrasound-induced increase in mutation to 6-TG resistance in in-vitro mammalian cells.

AB - In-vitro V79 and L5178Y cells were exposed in a rotating test tube to continuous-wave (CW) 1 MHz 35 W/cm2 ultrasound (0-4 or 0-3 min, respectively) and subsequently assayed for mutation as evidenced by resistance to 6-thioguanine (6-TG). There was a modest but statistically significant increase in mutation frequency in both cell types with increase in ultrasound exposure duration. X-ray exposures (3-9 Gy, a "positive control") yielded a large increase in 6-TG resistance. The data support an earlier report by Kaufman (1985) of an ultrasound-induced increase in mutation to 6-TG resistance in in-vitro mammalian cells.

KW - Acoustics

KW - Cell mutation

KW - In-vitro mammalian cells

KW - Ultrasound

KW - X-rays

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ER -

Confirmation of an ultrasound-induced mutation in two in-vitro mammalian cell lines

Abstract

Keywords

ASJC Scopus subject areas

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