Confirmation of an ultrasound-induced mutation in two in-vitro mammalian cell lines

Yukio Doida, Morton W. Miller, Christopher Cox, Charles C. Church

Research output: Contribution to journalArticlepeer-review

Abstract

In-vitro V79 and L5178Y cells were exposed in a rotating test tube to continuous-wave (CW) 1 MHz 35 W/cm2 ultrasound (0-4 or 0-3 min, respectively) and subsequently assayed for mutation as evidenced by resistance to 6-thioguanine (6-TG). There was a modest but statistically significant increase in mutation frequency in both cell types with increase in ultrasound exposure duration. X-ray exposures (3-9 Gy, a "positive control") yielded a large increase in 6-TG resistance. The data support an earlier report by Kaufman (1985) of an ultrasound-induced increase in mutation to 6-TG resistance in in-vitro mammalian cells.

Original languageEnglish (US)
Pages (from-to)699-705
Number of pages7
JournalUltrasound in Medicine and Biology
Volume16
Issue number7
DOIs
StatePublished - 1990
Externally publishedYes

Keywords

  • Acoustics
  • Cell mutation
  • In-vitro mammalian cells
  • Ultrasound
  • X-rays

ASJC Scopus subject areas

  • Radiological and Ultrasound Technology
  • Biophysics
  • Acoustics and Ultrasonics

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