Conditional sigma factor expression, using the inducible acetamidase promoter, reveals that the Mycobacterium tuberculosis sigF gene modulates expression of the 16-kilodalton alpha-crystallin homologue

Yukari C. Manabe, Jong Min Chen, Chiew G. Ko, Ping Chen, William R. Bishai

Research output: Contribution to journalArticle

Abstract

A chemically inducible acetamidase promoter-sigF fusion gene was integrated into the chromosome of Mycobacterium bovis BCG. Two-dimensional protein gel analysis permitted the identification of a number of protein spots whose expression was SigF related. One spot upregulated by inappropriate induction of sigF expression corresponded to the 16-kDa antigen alpha-crystallin.

Original languageEnglish (US)
Pages (from-to)7629-7633
Number of pages5
JournalJournal of bacteriology
Volume181
Issue number24
DOIs
StatePublished - Dec 1999

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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