Conditional sigma factor expression, using the inducible acetamidase promoter, reveals that the Mycobacterium tuberculosis sigF gene modulates expression of the 16-kilodalton alpha-crystallin homologue

Yukari C. Manabe; Jong Min Chen; Chiew G. Ko; Ping Chen; William R. Bishai

doi:10.1128/jb.181.24.7629-7633.1999

Conditional sigma factor expression, using the inducible acetamidase promoter, reveals that the Mycobacterium tuberculosis sigF gene modulates expression of the 16-kilodalton alpha-crystallin homologue

Yukari C. Manabe, Jong Min Chen, Chiew G. Ko, Ping Chen, William R. Bishai

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

A chemically inducible acetamidase promoter-sigF fusion gene was integrated into the chromosome of Mycobacterium bovis BCG. Two-dimensional protein gel analysis permitted the identification of a number of protein spots whose expression was SigF related. One spot upregulated by inappropriate induction of sigF expression corresponded to the 16-kDa antigen alpha-crystallin.

Original language	English (US)
Pages (from-to)	7629-7633
Number of pages	5
Journal	Journal of bacteriology
Volume	181
Issue number	24
DOIs	https://doi.org/10.1128/jb.181.24.7629-7633.1999
State	Published - Dec 1999

ASJC Scopus subject areas

Microbiology
Molecular Biology

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10.1128/jb.181.24.7629-7633.1999

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Conditional sigma factor expression, using the inducible acetamidase promoter, reveals that the Mycobacterium tuberculosis sigF gene modulates expression of the 16-kilodalton alpha-crystallin homologue. / Manabe, Yukari C.; Chen, Jong Min; Ko, Chiew G. et al.
In: Journal of bacteriology, Vol. 181, No. 24, 12.1999, p. 7629-7633.

Research output: Contribution to journal › Article › peer-review

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Conditional sigma factor expression, using the inducible acetamidase promoter, reveals that the Mycobacterium tuberculosis sigF gene modulates expression of the 16-kilodalton alpha-crystallin homologue

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