@inbook{dffdfa80e30c474bbff8703435b328c4,
title = "Conditional mutation of SMC5 in mouse embryonic fibroblasts",
abstract = "The structural maintenance of chromosomes (SMC) complex, SMC5/6, is important for genome maintenance in all model eukaryotes. To date, the most extensive studies have focused on the roles of Smc5/6 in lower eukaryotes, such as yeast and fly. In the handful of studies that have used mammalian cells, siRNA was used by most to knockdown SMC5/6 components. RNAi methods have been very important for scientific progression, but they are hindered by incomplete silencing of protein expression and off-target effects. This chapter outlines the use of a conditional knockout approach in mouse embryonic fibroblasts to study the function of the SMC5/6 complex. These cell lines provide an alternative method to study the function and properties of the SMC5/6 complex in mammals.",
keywords = "Chromosome segregation, Conditional knockout, DNA bridges, DNA damage, Micronuclei, Mitotic catastrophe, Mouse embryonic fibroblast, SMC5/6, Structural maintenance of chromosomes",
author = "Himaja Gaddipati and Pryzhkova, {Marina V.} and Jordan, {Philip W.}",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2019.",
year = "2019",
doi = "10.1007/978-1-4939-9520-2_4",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "35--46",
booktitle = "Methods in Molecular Biology",
}