Conditional mutation of SMC5 in mouse embryonic fibroblasts

Himaja Gaddipati, Marina Pryzhkova, Philip Jordan

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The structural maintenance of chromosomes (SMC) complex, SMC5/6, is important for genome maintenance in all model eukaryotes. To date, the most extensive studies have focused on the roles of Smc5/6 in lower eukaryotes, such as yeast and fly. In the handful of studies that have used mammalian cells, siRNA was used by most to knockdown SMC5/6 components. RNAi methods have been very important for scientific progression, but they are hindered by incomplete silencing of protein expression and off-target effects. This chapter outlines the use of a conditional knockout approach in mouse embryonic fibroblasts to study the function of the SMC5/6 complex. These cell lines provide an alternative method to study the function and properties of the SMC5/6 complex in mammals.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages35-46
Number of pages12
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume2004
ISSN (Print)1064-3745

Keywords

  • Chromosome segregation
  • Conditional knockout
  • DNA bridges
  • DNA damage
  • Micronuclei
  • Mitotic catastrophe
  • Mouse embryonic fibroblast
  • SMC5/6
  • Structural maintenance of chromosomes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Gaddipati, H., Pryzhkova, M., & Jordan, P. (2019). Conditional mutation of SMC5 in mouse embryonic fibroblasts. In Methods in Molecular Biology (pp. 35-46). (Methods in Molecular Biology; Vol. 2004). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9520-2_4