Concurrent responses of peripheral blood and splenic mononuclear cells to antigenic and mitogenic stimulation in human hepatosplenic schistosomiasis

Neil E. Reiner, Rifaat Kamel, Gene I. Higashi, Adel El Naggar, Mounir Aguib, Jerrold J. Ellner, Adel A F Mahmoud

Research output: Contribution to journalArticle

Abstract

Lymphocyte reactivity and its control was assessed in human hepatosplenic schistosomiasis, a disease in which host hypersensitivity may contribute to long-term morbidity. Antigen-and mitogen-induced incorporation of [3H]thymidine was evaluated in peripheral blood and splenic mononuclear cells of 15 patients at the time of splenectomy. The response to phytohemagglutinin (PHA) was depressed in the peripheral blood of 42% and in the spleen cells of 70% of these patients. Significant stimulation was noted, however, upon culture of blood with soluble schistosome egg antigen (SEA) in 80% and of spleen cells in 100% of the patients whose respective responses to PHA were depressed. The contribution of adherent cells to the overall response of mononuclear cells was evaluated by depletion techniques. A significant and specific decrease in the response of the resulting thymus-derived (T) lymphocyte-enriched splenic mononuclear cells to SEA was noted. These studies suggest preferential preservation of the response of circulating and splenic lymphocytes to SEA despite impairment. of PHA reactivity in human hepatosplenic schistosomiasis, which may be causally related to the advanced disease of this group of patients. Moreover, activity of helper adherent cells was consistently restricted to the splenic T-lymphocyte response induced by the specific antigen SEA.

Original languageEnglish (US)
Pages (from-to)162-168
Number of pages7
JournalJournal of Infectious Diseases
Volume140
Issue number2
DOIs
StatePublished - 1979
Externally publishedYes

Fingerprint

Schistosomiasis
Antigens
Ovum
Phytohemagglutinins
Spleen
Lymphocytes
T-Lymphocytes
Splenectomy
Helper-Inducer T-Lymphocytes
Mitogens
Thymidine
Thymus Gland
Hypersensitivity
Morbidity

ASJC Scopus subject areas

  • Infectious Diseases
  • Immunology and Allergy
  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Concurrent responses of peripheral blood and splenic mononuclear cells to antigenic and mitogenic stimulation in human hepatosplenic schistosomiasis. / Reiner, Neil E.; Kamel, Rifaat; Higashi, Gene I.; El Naggar, Adel; Aguib, Mounir; Ellner, Jerrold J.; Mahmoud, Adel A F.

In: Journal of Infectious Diseases, Vol. 140, No. 2, 1979, p. 162-168.

Research output: Contribution to journalArticle

Reiner, Neil E. ; Kamel, Rifaat ; Higashi, Gene I. ; El Naggar, Adel ; Aguib, Mounir ; Ellner, Jerrold J. ; Mahmoud, Adel A F. / Concurrent responses of peripheral blood and splenic mononuclear cells to antigenic and mitogenic stimulation in human hepatosplenic schistosomiasis. In: Journal of Infectious Diseases. 1979 ; Vol. 140, No. 2. pp. 162-168.
@article{f195a4a85dc44fa3b84e25897ea64b2e,
title = "Concurrent responses of peripheral blood and splenic mononuclear cells to antigenic and mitogenic stimulation in human hepatosplenic schistosomiasis",
abstract = "Lymphocyte reactivity and its control was assessed in human hepatosplenic schistosomiasis, a disease in which host hypersensitivity may contribute to long-term morbidity. Antigen-and mitogen-induced incorporation of [3H]thymidine was evaluated in peripheral blood and splenic mononuclear cells of 15 patients at the time of splenectomy. The response to phytohemagglutinin (PHA) was depressed in the peripheral blood of 42{\%} and in the spleen cells of 70{\%} of these patients. Significant stimulation was noted, however, upon culture of blood with soluble schistosome egg antigen (SEA) in 80{\%} and of spleen cells in 100{\%} of the patients whose respective responses to PHA were depressed. The contribution of adherent cells to the overall response of mononuclear cells was evaluated by depletion techniques. A significant and specific decrease in the response of the resulting thymus-derived (T) lymphocyte-enriched splenic mononuclear cells to SEA was noted. These studies suggest preferential preservation of the response of circulating and splenic lymphocytes to SEA despite impairment. of PHA reactivity in human hepatosplenic schistosomiasis, which may be causally related to the advanced disease of this group of patients. Moreover, activity of helper adherent cells was consistently restricted to the splenic T-lymphocyte response induced by the specific antigen SEA.",
author = "Reiner, {Neil E.} and Rifaat Kamel and Higashi, {Gene I.} and {El Naggar}, Adel and Mounir Aguib and Ellner, {Jerrold J.} and Mahmoud, {Adel A F}",
year = "1979",
doi = "10.1093/infdis/140.2.162",
language = "English (US)",
volume = "140",
pages = "162--168",
journal = "Journal of Infectious Diseases",
issn = "0022-1899",
publisher = "Oxford University Press",
number = "2",

}

TY - JOUR

T1 - Concurrent responses of peripheral blood and splenic mononuclear cells to antigenic and mitogenic stimulation in human hepatosplenic schistosomiasis

AU - Reiner, Neil E.

AU - Kamel, Rifaat

AU - Higashi, Gene I.

AU - El Naggar, Adel

AU - Aguib, Mounir

AU - Ellner, Jerrold J.

AU - Mahmoud, Adel A F

PY - 1979

Y1 - 1979

N2 - Lymphocyte reactivity and its control was assessed in human hepatosplenic schistosomiasis, a disease in which host hypersensitivity may contribute to long-term morbidity. Antigen-and mitogen-induced incorporation of [3H]thymidine was evaluated in peripheral blood and splenic mononuclear cells of 15 patients at the time of splenectomy. The response to phytohemagglutinin (PHA) was depressed in the peripheral blood of 42% and in the spleen cells of 70% of these patients. Significant stimulation was noted, however, upon culture of blood with soluble schistosome egg antigen (SEA) in 80% and of spleen cells in 100% of the patients whose respective responses to PHA were depressed. The contribution of adherent cells to the overall response of mononuclear cells was evaluated by depletion techniques. A significant and specific decrease in the response of the resulting thymus-derived (T) lymphocyte-enriched splenic mononuclear cells to SEA was noted. These studies suggest preferential preservation of the response of circulating and splenic lymphocytes to SEA despite impairment. of PHA reactivity in human hepatosplenic schistosomiasis, which may be causally related to the advanced disease of this group of patients. Moreover, activity of helper adherent cells was consistently restricted to the splenic T-lymphocyte response induced by the specific antigen SEA.

AB - Lymphocyte reactivity and its control was assessed in human hepatosplenic schistosomiasis, a disease in which host hypersensitivity may contribute to long-term morbidity. Antigen-and mitogen-induced incorporation of [3H]thymidine was evaluated in peripheral blood and splenic mononuclear cells of 15 patients at the time of splenectomy. The response to phytohemagglutinin (PHA) was depressed in the peripheral blood of 42% and in the spleen cells of 70% of these patients. Significant stimulation was noted, however, upon culture of blood with soluble schistosome egg antigen (SEA) in 80% and of spleen cells in 100% of the patients whose respective responses to PHA were depressed. The contribution of adherent cells to the overall response of mononuclear cells was evaluated by depletion techniques. A significant and specific decrease in the response of the resulting thymus-derived (T) lymphocyte-enriched splenic mononuclear cells to SEA was noted. These studies suggest preferential preservation of the response of circulating and splenic lymphocytes to SEA despite impairment. of PHA reactivity in human hepatosplenic schistosomiasis, which may be causally related to the advanced disease of this group of patients. Moreover, activity of helper adherent cells was consistently restricted to the splenic T-lymphocyte response induced by the specific antigen SEA.

UR - http://www.scopus.com/inward/record.url?scp=0018720023&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018720023&partnerID=8YFLogxK

U2 - 10.1093/infdis/140.2.162

DO - 10.1093/infdis/140.2.162

M3 - Article

C2 - 314479

AN - SCOPUS:0018720023

VL - 140

SP - 162

EP - 168

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

SN - 0022-1899

IS - 2

ER -