Concordance of human papillomavirus in the cervix and urine among inner city adolescents

Denise L. Jacobson, Sharita D. Womack, Ligia Peralta, Jonathan Mark Zenilman, Kathy Feroli, Jennifer Maehr, Richard W. Daniel, Keerti V. Shah

Research output: Contribution to journalArticle

Abstract

Objectives. To evaluate concordance for human papillomaviruses (HPVs) between cervix and urine in sexually active adolescents. Methods. Cervical swabs and urine were collected from 80 adolescents in Baltimore, MD. Specimens were tested for 34 HPVs by PCR and for cancer-associated HPVs by Hybrid Capture (HC II) Probe B. Cervical vs. urine prevalence was evaluated by logistic regression with general estimating equations. Risk factors for prevalence and viral burden were evaluated by Fisher's exact and Kruskal-Wallis tests, respectively. Results. HPV prevalence by PCR, for any HPV, was very high in the cervix (90.0%) and somewhat lower in urine (75.0%) (odds ratio, 1.07; 95% confidence interval 1.07 to 1.34). Only one adolescent was HPV-positive in urine alone. Among HPV-PCR positives at either or both sites, concordance was 82% for presence of any HPV and 40% for specific HPV types. Multiple infections were common at both sites. On an average, HC II viral burden (relative light unit ratio) was 9-fold higher in cervix than in urine (median, 47.3 vs. 4.9; P = 0.005) but correlated poorly between the two sites of the same individual (r = 0.14). Compared with normal adolescents, those with squamous intraepithelial lesions had a much higher prevalence of HPV by HC II in cervix (100% vs. 28.6, P <0.0001) as well as in urine (86.7% vs. 35.4%, P = 0.002) and a significantly higher viral burden in the cervix (median, 141.8 vs. 7.3, P = 0.0045) but not in urine (median, 22.7 vs. 4.38; P = 0.13). Conclusion. There was a very high prevalence of HPV in cervix and urine of sexually active adolescents. Testing urine for HPV may be useful in epidemiologic investigations and in monitoring of infected women.

Original languageEnglish (US)
Pages (from-to)722-728
Number of pages7
JournalPediatric Infectious Disease Journal
Volume19
Issue number8
DOIs
StatePublished - 2000

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Cervix Uteri
Urine
Viral Load
Polymerase Chain Reaction
Baltimore
Logistic Models
Odds Ratio
Confidence Intervals
Light

Keywords

  • Adolescents
  • Cervix
  • Concordance
  • Human papillomavirus
  • Prevalence
  • Urine

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Microbiology (medical)

Cite this

Concordance of human papillomavirus in the cervix and urine among inner city adolescents. / Jacobson, Denise L.; Womack, Sharita D.; Peralta, Ligia; Zenilman, Jonathan Mark; Feroli, Kathy; Maehr, Jennifer; Daniel, Richard W.; Shah, Keerti V.

In: Pediatric Infectious Disease Journal, Vol. 19, No. 8, 2000, p. 722-728.

Research output: Contribution to journalArticle

Jacobson, Denise L. ; Womack, Sharita D. ; Peralta, Ligia ; Zenilman, Jonathan Mark ; Feroli, Kathy ; Maehr, Jennifer ; Daniel, Richard W. ; Shah, Keerti V. / Concordance of human papillomavirus in the cervix and urine among inner city adolescents. In: Pediatric Infectious Disease Journal. 2000 ; Vol. 19, No. 8. pp. 722-728.
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abstract = "Objectives. To evaluate concordance for human papillomaviruses (HPVs) between cervix and urine in sexually active adolescents. Methods. Cervical swabs and urine were collected from 80 adolescents in Baltimore, MD. Specimens were tested for 34 HPVs by PCR and for cancer-associated HPVs by Hybrid Capture (HC II) Probe B. Cervical vs. urine prevalence was evaluated by logistic regression with general estimating equations. Risk factors for prevalence and viral burden were evaluated by Fisher's exact and Kruskal-Wallis tests, respectively. Results. HPV prevalence by PCR, for any HPV, was very high in the cervix (90.0{\%}) and somewhat lower in urine (75.0{\%}) (odds ratio, 1.07; 95{\%} confidence interval 1.07 to 1.34). Only one adolescent was HPV-positive in urine alone. Among HPV-PCR positives at either or both sites, concordance was 82{\%} for presence of any HPV and 40{\%} for specific HPV types. Multiple infections were common at both sites. On an average, HC II viral burden (relative light unit ratio) was 9-fold higher in cervix than in urine (median, 47.3 vs. 4.9; P = 0.005) but correlated poorly between the two sites of the same individual (r = 0.14). Compared with normal adolescents, those with squamous intraepithelial lesions had a much higher prevalence of HPV by HC II in cervix (100{\%} vs. 28.6, P <0.0001) as well as in urine (86.7{\%} vs. 35.4{\%}, P = 0.002) and a significantly higher viral burden in the cervix (median, 141.8 vs. 7.3, P = 0.0045) but not in urine (median, 22.7 vs. 4.38; P = 0.13). Conclusion. There was a very high prevalence of HPV in cervix and urine of sexually active adolescents. Testing urine for HPV may be useful in epidemiologic investigations and in monitoring of infected women.",
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AU - Peralta, Ligia

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AU - Feroli, Kathy

AU - Maehr, Jennifer

AU - Daniel, Richard W.

AU - Shah, Keerti V.

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N2 - Objectives. To evaluate concordance for human papillomaviruses (HPVs) between cervix and urine in sexually active adolescents. Methods. Cervical swabs and urine were collected from 80 adolescents in Baltimore, MD. Specimens were tested for 34 HPVs by PCR and for cancer-associated HPVs by Hybrid Capture (HC II) Probe B. Cervical vs. urine prevalence was evaluated by logistic regression with general estimating equations. Risk factors for prevalence and viral burden were evaluated by Fisher's exact and Kruskal-Wallis tests, respectively. Results. HPV prevalence by PCR, for any HPV, was very high in the cervix (90.0%) and somewhat lower in urine (75.0%) (odds ratio, 1.07; 95% confidence interval 1.07 to 1.34). Only one adolescent was HPV-positive in urine alone. Among HPV-PCR positives at either or both sites, concordance was 82% for presence of any HPV and 40% for specific HPV types. Multiple infections were common at both sites. On an average, HC II viral burden (relative light unit ratio) was 9-fold higher in cervix than in urine (median, 47.3 vs. 4.9; P = 0.005) but correlated poorly between the two sites of the same individual (r = 0.14). Compared with normal adolescents, those with squamous intraepithelial lesions had a much higher prevalence of HPV by HC II in cervix (100% vs. 28.6, P <0.0001) as well as in urine (86.7% vs. 35.4%, P = 0.002) and a significantly higher viral burden in the cervix (median, 141.8 vs. 7.3, P = 0.0045) but not in urine (median, 22.7 vs. 4.38; P = 0.13). Conclusion. There was a very high prevalence of HPV in cervix and urine of sexually active adolescents. Testing urine for HPV may be useful in epidemiologic investigations and in monitoring of infected women.

AB - Objectives. To evaluate concordance for human papillomaviruses (HPVs) between cervix and urine in sexually active adolescents. Methods. Cervical swabs and urine were collected from 80 adolescents in Baltimore, MD. Specimens were tested for 34 HPVs by PCR and for cancer-associated HPVs by Hybrid Capture (HC II) Probe B. Cervical vs. urine prevalence was evaluated by logistic regression with general estimating equations. Risk factors for prevalence and viral burden were evaluated by Fisher's exact and Kruskal-Wallis tests, respectively. Results. HPV prevalence by PCR, for any HPV, was very high in the cervix (90.0%) and somewhat lower in urine (75.0%) (odds ratio, 1.07; 95% confidence interval 1.07 to 1.34). Only one adolescent was HPV-positive in urine alone. Among HPV-PCR positives at either or both sites, concordance was 82% for presence of any HPV and 40% for specific HPV types. Multiple infections were common at both sites. On an average, HC II viral burden (relative light unit ratio) was 9-fold higher in cervix than in urine (median, 47.3 vs. 4.9; P = 0.005) but correlated poorly between the two sites of the same individual (r = 0.14). Compared with normal adolescents, those with squamous intraepithelial lesions had a much higher prevalence of HPV by HC II in cervix (100% vs. 28.6, P <0.0001) as well as in urine (86.7% vs. 35.4%, P = 0.002) and a significantly higher viral burden in the cervix (median, 141.8 vs. 7.3, P = 0.0045) but not in urine (median, 22.7 vs. 4.38; P = 0.13). Conclusion. There was a very high prevalence of HPV in cervix and urine of sexually active adolescents. Testing urine for HPV may be useful in epidemiologic investigations and in monitoring of infected women.

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