Concise review: Human cell engineering: Cellular reprogramming and genome editing

Prashant Mali, Linzhao Cheng

Research output: Contribution to journalReview article

Abstract

Cell engineering is defined here as the collective ability to both reset and edit the genome of a mammalian cell. Until recently, this had been extremely challenging to achieve as nontransformed human cells are significantly refractory to both these processes. The recent success in reprogramming somatic cells into induced pluripotent stem cells that are self-renewable in culture, coupled with our increasing ability to effect precise and predesigned genomic editing, now readily permits cellular changes at both the genetic and epigenetic levels. These dual capabilities also make possible the generation of genetically matched, disease-free stem cells from patients for regenerative medicine. The objective of this review is to summarize the key enabling developments on these two rapidly evolving research fronts in human cell engineering, highlight unresolved issues, and outline potential future research directions.

Original languageEnglish (US)
Pages (from-to)75-81
Number of pages7
JournalStem Cells
Volume30
Issue number1
DOIs
StatePublished - Jan 2012

Keywords

  • Disease modeling
  • Gene targeting
  • Gene therapy
  • Genome editing
  • Genome engineering
  • Human cell reprogramming
  • Pluripotent stem cells

ASJC Scopus subject areas

  • Molecular Medicine
  • Developmental Biology
  • Cell Biology

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