Concepts related to salt resistant estradiol receptors in rat uterine nuclei: Nuclear matrix

Evelyn R. Barrack; Edward F. Hawkins; Steven L. Allen; L. Louise Hicks; Donald S. Coffey

doi:10.1016/0006-291X(77)91186-X

Concepts related to salt resistant estradiol receptors in rat uterine nuclei: Nuclear matrix

Evelyn R. Barrack, Edward F. Hawkins, Steven L. Allen, L. Louise Hicks, Donald S. Coffey

School of Medicine

Research output: Contribution to journal › Article › peer-review

49 Scopus citations

Abstract

When ³H-estradiol (0.1 μg) is injected into immature female rats, virtually all of the label that is recovered with uterine nuclei can be solubilized by 0.6 M KCl. Salt resistant uterine nuclear estrogen binding sites do not become labeled within one hour after the injection of ³H-estradiol, but these sites do exist and can be revealed when isolated nuclei are subjected to an in vitro estradiol exchange assay. These saturable, high affinity salt resistant sites appear to be associated with the uterine nuclear matrix, a residual structure of the nucleus.

Original language	English (US)
Pages (from-to)	829-836
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	79
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(77)91186-X
State	Published - Dec 7 1977

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0006-291X(77)91186-X

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abstract = "When 3H-estradiol (0.1 μg) is injected into immature female rats, virtually all of the label that is recovered with uterine nuclei can be solubilized by 0.6 M KCl. Salt resistant uterine nuclear estrogen binding sites do not become labeled within one hour after the injection of 3H-estradiol, but these sites do exist and can be revealed when isolated nuclei are subjected to an in vitro estradiol exchange assay. These saturable, high affinity salt resistant sites appear to be associated with the uterine nuclear matrix, a residual structure of the nucleus.",

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AU - Hawkins, Edward F.

AU - Allen, Steven L.

AU - Louise Hicks, L.

AU - Coffey, Donald S.

PY - 1977/12/7

Y1 - 1977/12/7

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AB - When 3H-estradiol (0.1 μg) is injected into immature female rats, virtually all of the label that is recovered with uterine nuclei can be solubilized by 0.6 M KCl. Salt resistant uterine nuclear estrogen binding sites do not become labeled within one hour after the injection of 3H-estradiol, but these sites do exist and can be revealed when isolated nuclei are subjected to an in vitro estradiol exchange assay. These saturable, high affinity salt resistant sites appear to be associated with the uterine nuclear matrix, a residual structure of the nucleus.

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Concepts related to salt resistant estradiol receptors in rat uterine nuclei: Nuclear matrix

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