Computer programming for nucleic acid studies III. Calculated ultraviolet absorption spectra of protected oligodeoxyribonucleotides

Lou sing Kan, Ralph W. Kettell, Paul S. Miller

Research output: Contribution to journalArticle

Abstract

A computer program called UV.FOR was written in FORTRAN. This program primarily utilizes the digitized UV absorption spectra of 8 protected deoxyribonucleosides in 95% ethanol solution to compose the UV spectrum of a oligodeoxynucleotide of any sequence. Both calculated and observed UV spectra of 2 protected oligodeoxynucleotides are carefully compared. The results show that the calculated UV spectrum is virtually identical to the observed spectrum. Thus, the calculated spectra provide rapid confirmation of oligonucleotide compositions during the course of oligonucleotide synthesis by the phosphotriester method.

Original languageEnglish (US)
Pages (from-to)185-190
Number of pages6
JournalComputer Programs in Biomedicine
Volume13
Issue number3-4
DOIs
Publication statusPublished - 1981

    Fingerprint

Keywords

  • Absorption spectrum
  • Oligonucleotides
  • Ultraviolet

ASJC Scopus subject areas

  • Medicine (miscellaneous)

Cite this