Computational and experimental analysis reveals a novel Src family kinase in the C.elegans genome

Akhilesh Pandey, Suraj Peri, Colin Thacker, Cherry A. Whipple, John J. Collins, Matthias Mann

Research output: Contribution to journalArticle

Abstract

Motivation: The complete genomes of a number of organisms have already been sequenced. However, the vast majority of annotated genes are derived by gene prediction methods. It is important to not only validate the predicted coding regions but also to identify genes that may have been missed by these programs. Methods: We searched the entire C.elegans genomic sequence database maintained by the Sanger Center using human c-Src sequence in a TBLASN search. We have confirmed one of the predicted regions by isolation of a cDNA and carried out a phylogenetic analysis of Src kinase family members in the worm, fly and several vertebrate species. Results: Our analysis identified a novel tyrosine kinase in the C.elegans genome that contains functional features typical of the Src family kinases that we have designated as Src-1. The open reading frame contains a conserved N-terminal myristoylation site and a tyrosine residue within the C-terminus that is crucial for regulating the activity of Src kinases. Our phylogenetic analysis of Src family members from C.elegans, Drosophila and other higher organisms revealed a relationship among Src kinases from C.elegans and Drosophila.

Original languageEnglish (US)
Pages (from-to)169-172
Number of pages4
JournalBioinformatics
Volume19
Issue number2
DOIs
StatePublished - Feb 1 2003
Externally publishedYes

Fingerprint

src-Family Kinases
Computational Analysis
Experimental Analysis
Phylogenetic Analysis
Genome
Genes
Drosophilidae
Gene
Drosophila
Worm
CDNA
Isolation
Genomics
Coding
Diptera
Entire
Protein-Tyrosine Kinases
Open Reading Frames
Tyrosine
Vertebrates

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Computer Science Applications
  • Computational Theory and Mathematics

Cite this

Pandey, A., Peri, S., Thacker, C., Whipple, C. A., Collins, J. J., & Mann, M. (2003). Computational and experimental analysis reveals a novel Src family kinase in the C.elegans genome. Bioinformatics, 19(2), 169-172. https://doi.org/10.1093/bioinformatics/19.2.169

Computational and experimental analysis reveals a novel Src family kinase in the C.elegans genome. / Pandey, Akhilesh; Peri, Suraj; Thacker, Colin; Whipple, Cherry A.; Collins, John J.; Mann, Matthias.

In: Bioinformatics, Vol. 19, No. 2, 01.02.2003, p. 169-172.

Research output: Contribution to journalArticle

Pandey, A, Peri, S, Thacker, C, Whipple, CA, Collins, JJ & Mann, M 2003, 'Computational and experimental analysis reveals a novel Src family kinase in the C.elegans genome', Bioinformatics, vol. 19, no. 2, pp. 169-172. https://doi.org/10.1093/bioinformatics/19.2.169
Pandey, Akhilesh ; Peri, Suraj ; Thacker, Colin ; Whipple, Cherry A. ; Collins, John J. ; Mann, Matthias. / Computational and experimental analysis reveals a novel Src family kinase in the C.elegans genome. In: Bioinformatics. 2003 ; Vol. 19, No. 2. pp. 169-172.
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