Comprehensive proteomic analysis of human pancreatic juice

Mads Grønborg, Jakob Bunkenborg, Troels Zakarias Kristiansen, Ole Nørregaard Jensen, Charles J. Yeo, Ralph H Hruban, Anirban Maitra, Michael S Goggins, Akhilesh Pandey

Research output: Contribution to journalArticle

Abstract

Proteomic technologies provide an excellent means for analysis of body fluids for cataloging protein constituents and identifying biomarkers for early detection of cancers. The biomarkers currently available for pancreatic cancer, such as CA19-9, lack adequate sensitivity and specificity contributing to late diagnosis of this deadly disease. In this study, we carried out a comprehensive characterization of the "pancreatic juice proteome" in patients with pancreatic adenocarcinoma. Pancreatic juice was first fractionated by 1-dimensional gel electrophoresis and subsequently analyzed by liquid chromatography tandem mass spectrometry (LC-MS/MS). A total of 170 unique proteins were identified including known pancreatic cancer tumor markers (e.g., CEA, MUC1) and proteins overexpressed in pancreatic cancers (e.g., hepatocarcinoma-intestine-pancreas/pancreatitis-associated protein (HIP/PAP) and lipocalin 2). In addition, we identified a number of proteins that have not been previously described in pancreatic juice (e.g., tumor rejection antigen (pg96) and azurocidin). Interestingly, a novel protein that is 85% identical to HIP/PAP was identified, which we have designated as PAP-2. The proteins identified in this study could be directly assessed for their potential as biomarkers for pancreatic cancer by quantitative proteomics methods or immunoassays.

Original languageEnglish (US)
Pages (from-to)1042-1055
Number of pages14
JournalJournal of Proteome Research
Volume3
Issue number5
DOIs
StatePublished - Sep 2004

Fingerprint

Pancreatic Juice
Proteomics
Pancreatic Neoplasms
Biomarkers
Proteins
Intestines
Pancreas
Cataloging
Lipocalins
Delayed Diagnosis
Body fluids
Liquid chromatography
Body Fluids
Neoplasm Antigens
Proteome
Tumor Biomarkers
Tandem Mass Spectrometry
Electrophoresis
Early Detection of Cancer
Immunoassay

Keywords

  • Body fluid
  • Functional annotation
  • Liquid chromatography tandem mass spectrometry (LC-MS/MS)

ASJC Scopus subject areas

  • Genetics
  • Biotechnology
  • Biochemistry

Cite this

Grønborg, M., Bunkenborg, J., Kristiansen, T. Z., Jensen, O. N., Yeo, C. J., Hruban, R. H., ... Pandey, A. (2004). Comprehensive proteomic analysis of human pancreatic juice. Journal of Proteome Research, 3(5), 1042-1055. https://doi.org/10.1021/pr0499085

Comprehensive proteomic analysis of human pancreatic juice. / Grønborg, Mads; Bunkenborg, Jakob; Kristiansen, Troels Zakarias; Jensen, Ole Nørregaard; Yeo, Charles J.; Hruban, Ralph H; Maitra, Anirban; Goggins, Michael S; Pandey, Akhilesh.

In: Journal of Proteome Research, Vol. 3, No. 5, 09.2004, p. 1042-1055.

Research output: Contribution to journalArticle

Grønborg, M, Bunkenborg, J, Kristiansen, TZ, Jensen, ON, Yeo, CJ, Hruban, RH, Maitra, A, Goggins, MS & Pandey, A 2004, 'Comprehensive proteomic analysis of human pancreatic juice', Journal of Proteome Research, vol. 3, no. 5, pp. 1042-1055. https://doi.org/10.1021/pr0499085
Grønborg M, Bunkenborg J, Kristiansen TZ, Jensen ON, Yeo CJ, Hruban RH et al. Comprehensive proteomic analysis of human pancreatic juice. Journal of Proteome Research. 2004 Sep;3(5):1042-1055. https://doi.org/10.1021/pr0499085
Grønborg, Mads ; Bunkenborg, Jakob ; Kristiansen, Troels Zakarias ; Jensen, Ole Nørregaard ; Yeo, Charles J. ; Hruban, Ralph H ; Maitra, Anirban ; Goggins, Michael S ; Pandey, Akhilesh. / Comprehensive proteomic analysis of human pancreatic juice. In: Journal of Proteome Research. 2004 ; Vol. 3, No. 5. pp. 1042-1055.
@article{f35c3e0fe00f4c14a7087f64650967fd,
title = "Comprehensive proteomic analysis of human pancreatic juice",
abstract = "Proteomic technologies provide an excellent means for analysis of body fluids for cataloging protein constituents and identifying biomarkers for early detection of cancers. The biomarkers currently available for pancreatic cancer, such as CA19-9, lack adequate sensitivity and specificity contributing to late diagnosis of this deadly disease. In this study, we carried out a comprehensive characterization of the {"}pancreatic juice proteome{"} in patients with pancreatic adenocarcinoma. Pancreatic juice was first fractionated by 1-dimensional gel electrophoresis and subsequently analyzed by liquid chromatography tandem mass spectrometry (LC-MS/MS). A total of 170 unique proteins were identified including known pancreatic cancer tumor markers (e.g., CEA, MUC1) and proteins overexpressed in pancreatic cancers (e.g., hepatocarcinoma-intestine-pancreas/pancreatitis-associated protein (HIP/PAP) and lipocalin 2). In addition, we identified a number of proteins that have not been previously described in pancreatic juice (e.g., tumor rejection antigen (pg96) and azurocidin). Interestingly, a novel protein that is 85{\%} identical to HIP/PAP was identified, which we have designated as PAP-2. The proteins identified in this study could be directly assessed for their potential as biomarkers for pancreatic cancer by quantitative proteomics methods or immunoassays.",
keywords = "Body fluid, Functional annotation, Liquid chromatography tandem mass spectrometry (LC-MS/MS)",
author = "Mads Gr{\o}nborg and Jakob Bunkenborg and Kristiansen, {Troels Zakarias} and Jensen, {Ole N{\o}rregaard} and Yeo, {Charles J.} and Hruban, {Ralph H} and Anirban Maitra and Goggins, {Michael S} and Akhilesh Pandey",
year = "2004",
month = "9",
doi = "10.1021/pr0499085",
language = "English (US)",
volume = "3",
pages = "1042--1055",
journal = "Journal of Proteome Research",
issn = "1535-3893",
publisher = "American Chemical Society",
number = "5",

}

TY - JOUR

T1 - Comprehensive proteomic analysis of human pancreatic juice

AU - Grønborg, Mads

AU - Bunkenborg, Jakob

AU - Kristiansen, Troels Zakarias

AU - Jensen, Ole Nørregaard

AU - Yeo, Charles J.

AU - Hruban, Ralph H

AU - Maitra, Anirban

AU - Goggins, Michael S

AU - Pandey, Akhilesh

PY - 2004/9

Y1 - 2004/9

N2 - Proteomic technologies provide an excellent means for analysis of body fluids for cataloging protein constituents and identifying biomarkers for early detection of cancers. The biomarkers currently available for pancreatic cancer, such as CA19-9, lack adequate sensitivity and specificity contributing to late diagnosis of this deadly disease. In this study, we carried out a comprehensive characterization of the "pancreatic juice proteome" in patients with pancreatic adenocarcinoma. Pancreatic juice was first fractionated by 1-dimensional gel electrophoresis and subsequently analyzed by liquid chromatography tandem mass spectrometry (LC-MS/MS). A total of 170 unique proteins were identified including known pancreatic cancer tumor markers (e.g., CEA, MUC1) and proteins overexpressed in pancreatic cancers (e.g., hepatocarcinoma-intestine-pancreas/pancreatitis-associated protein (HIP/PAP) and lipocalin 2). In addition, we identified a number of proteins that have not been previously described in pancreatic juice (e.g., tumor rejection antigen (pg96) and azurocidin). Interestingly, a novel protein that is 85% identical to HIP/PAP was identified, which we have designated as PAP-2. The proteins identified in this study could be directly assessed for their potential as biomarkers for pancreatic cancer by quantitative proteomics methods or immunoassays.

AB - Proteomic technologies provide an excellent means for analysis of body fluids for cataloging protein constituents and identifying biomarkers for early detection of cancers. The biomarkers currently available for pancreatic cancer, such as CA19-9, lack adequate sensitivity and specificity contributing to late diagnosis of this deadly disease. In this study, we carried out a comprehensive characterization of the "pancreatic juice proteome" in patients with pancreatic adenocarcinoma. Pancreatic juice was first fractionated by 1-dimensional gel electrophoresis and subsequently analyzed by liquid chromatography tandem mass spectrometry (LC-MS/MS). A total of 170 unique proteins were identified including known pancreatic cancer tumor markers (e.g., CEA, MUC1) and proteins overexpressed in pancreatic cancers (e.g., hepatocarcinoma-intestine-pancreas/pancreatitis-associated protein (HIP/PAP) and lipocalin 2). In addition, we identified a number of proteins that have not been previously described in pancreatic juice (e.g., tumor rejection antigen (pg96) and azurocidin). Interestingly, a novel protein that is 85% identical to HIP/PAP was identified, which we have designated as PAP-2. The proteins identified in this study could be directly assessed for their potential as biomarkers for pancreatic cancer by quantitative proteomics methods or immunoassays.

KW - Body fluid

KW - Functional annotation

KW - Liquid chromatography tandem mass spectrometry (LC-MS/MS)

UR - http://www.scopus.com/inward/record.url?scp=7044237450&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=7044237450&partnerID=8YFLogxK

U2 - 10.1021/pr0499085

DO - 10.1021/pr0499085

M3 - Article

C2 - 15473694

AN - SCOPUS:7044237450

VL - 3

SP - 1042

EP - 1055

JO - Journal of Proteome Research

JF - Journal of Proteome Research

SN - 1535-3893

IS - 5

ER -