TY - JOUR
T1 - Comprehensive characterization of surface-functionalized poly(amidoamine) dendrimers with acetamide, hydroxyl, and carboxyl groups
AU - Shi, Xiangyang
AU - Lesniak, Wojciech
AU - Islam, Mohammad T.
AU - MuÑiz, Maria C.
AU - Balogh, Lajos P.
AU - Baker, James R.
N1 - Funding Information:
This work is financially supported by the Environmental Protection Agency, EPA Nanotechnology Award R829626 as part of the STAR program, the National Cancer Institute (NCI) under the contract # NOI-CO-97111 and the DOD Prostate Cancer program under contract # (DAMD17-02-1-0096).
PY - 2006/1/5
Y1 - 2006/1/5
N2 - Terminal amine groups of poly(amidoamine) (PAMAM) dendrimers can be substituted with different functional groups for various applications. In this study, PAMAM derivatives with acetamide, hydroxyl, and carboxyl termini were synthesized from ethylenediamine (EDA) core generation 4 and 5 primary amine-terminated PAMAM dendrimers. The reaction products were purified with dialysis and subsequently characterized by polyacrylamide gel electrophoresis (PAGE), capillary electrophoresis (CE), size exclusion chromatography (SEC), matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry, potentiometric titration, 1H NMR, and 13C NMR. PAGE and CE electropherograms provide data regarding the purity, charge distribution, and electrophoretic mobility of the dendrimers and their derivatives. SEC and MALDI-TOF mass spectrometry detect the average absolute molar mass and the individual mass fractions, respectively. The combination of SEC with potentiometric titration provides quantitative evidence of the degree of the functional group substitution, while NMR techniques (both 1H NMR and 13C NMR) confirmed the changes in dendrimer surface functionalization. This study provides a general example for the comprehensive characterization of surface-functionalized PAMAM dendrimer nanoparticles. The synthesized dendrimer derivatives hold promise for environmental and medical applications.
AB - Terminal amine groups of poly(amidoamine) (PAMAM) dendrimers can be substituted with different functional groups for various applications. In this study, PAMAM derivatives with acetamide, hydroxyl, and carboxyl termini were synthesized from ethylenediamine (EDA) core generation 4 and 5 primary amine-terminated PAMAM dendrimers. The reaction products were purified with dialysis and subsequently characterized by polyacrylamide gel electrophoresis (PAGE), capillary electrophoresis (CE), size exclusion chromatography (SEC), matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry, potentiometric titration, 1H NMR, and 13C NMR. PAGE and CE electropherograms provide data regarding the purity, charge distribution, and electrophoretic mobility of the dendrimers and their derivatives. SEC and MALDI-TOF mass spectrometry detect the average absolute molar mass and the individual mass fractions, respectively. The combination of SEC with potentiometric titration provides quantitative evidence of the degree of the functional group substitution, while NMR techniques (both 1H NMR and 13C NMR) confirmed the changes in dendrimer surface functionalization. This study provides a general example for the comprehensive characterization of surface-functionalized PAMAM dendrimer nanoparticles. The synthesized dendrimer derivatives hold promise for environmental and medical applications.
KW - Dendrimers
KW - Electrophoresis
KW - Functionalization of polymers
KW - Mass spectrometry
KW - Synthesis
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U2 - 10.1016/j.colsurfa.2005.07.031
DO - 10.1016/j.colsurfa.2005.07.031
M3 - Article
AN - SCOPUS:28844474501
SN - 0927-7757
VL - 272
SP - 139
EP - 150
JO - Colloids and Surfaces A: Physicochemical and Engineering Aspects
JF - Colloids and Surfaces A: Physicochemical and Engineering Aspects
IS - 1-2
ER -