Compositional control of pDNA/lPEI nanoparticles using flash nanocomplexation to improve in vivo transfection efficiency and biocompatibility

Yizong Hu, Heng Wen Liu, Il Minn, Martin Pomper, Hai Quan Mao

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Linear PEI (lPEI) has proven track record as an effective carrier for the delivery of therapeutic plasmid DNA (pDNA) for disease treatment. However, the lack of a reproducible method to produce pDNA/lPEI nanoparticles with controlled physicochemical characteristics and compositions has long hindered the development of these nanoparticles as a pharmaceutical product. We have employed a turbulent mixing methodology at high Reynolds numbers using the flash nanocomplexation (FNC) technique to achieve efficient and homogenous complexation of pDNA and lPEI. Through precise control of the kinetic conditions for the nanoparticle assembly, uniform nanoparticles with a well-defined composition was developed that presented benefit over both in vivo transfection efficiency and biocompatibility.

Original languageEnglish (US)
Title of host publicationSociety for Biomaterials Annual Meeting and Exposition 2019
Subtitle of host publicationThe Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting
PublisherSociety for Biomaterials
Number of pages1
ISBN (Electronic)9781510883901
StatePublished - Jan 1 2019
Event42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Seattle, United States
Duration: Apr 3 2019Apr 6 2019

Publication series

NameTransactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium
Volume40
ISSN (Print)1526-7547

Conference

Conference42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence
CountryUnited States
CitySeattle
Period4/3/194/6/19

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Biotechnology
  • Biomaterials
  • Materials Chemistry

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