Composition and distribution of low density lipoprotein fractions in hyperapobetalipoproteinemia, normolipidemia, and familial hypercholesterolemia

B. Teng, G. R. Thompson, A. D. Sniderman, T. M. Forte, R. M. Krauss, P. O. Kwiterovich

Research output: Contribution to journalArticle

Abstract

Hyperapobetalipoproteinemia is defined as the combination of a normal low density lipoprotein (LDL) cholesterol in face of an increased LDL apolipoprotein B (apoB) protein. To examine the physical basis for the apparent disproportion between LDL cholesterol and apoB characteristic of this syndrome, we used density gradient ultracentrifugation to isolate LDL fractions from 10 normal subjects, from 20 patients with hyperapobetalipoproteinemia (10 normotriglyceridemic and 10 hypertriglyceridemic), and from 7 patients with familial hypercholesterolemia. In familial hypercholesterolemia, more LDL was in fraction 1 - 'light' LDL - and this LDL was relatively enriched in cholesterol and poor in protein. By contrast, it was fraction 2 - 'heavy' LDL - that differed in hyperapobetalipoproteinemia, being denser, depleted of cholesterol (particularly cholesteryl ester), and relatively enriched in apoB. These findings were more pronounced in the hypertriglyceridemic patients than in the normotriglyceridemic patients with hyperapobetalipoproteinemia. Thus this study confirms that considerable heterogeneity exists between LDL subfractions within individuals but, in addition, indicates there also marked - and apparently characteristic - differences in LDL composition amongst normal subjects and patients with hyperapobetalipoproteinemia or familial hypercholesterolemia.

Original languageEnglish (US)
Pages (from-to)6662-6666
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume80
Issue number21 I
StatePublished - 1983
Externally publishedYes

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Hyperlipoproteinemia Type II
LDL Lipoproteins
Apolipoproteins B
LDL Cholesterol
Cholesterol
Cholesterol Esters
Ultracentrifugation
Proteins

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Composition and distribution of low density lipoprotein fractions in hyperapobetalipoproteinemia, normolipidemia, and familial hypercholesterolemia. / Teng, B.; Thompson, G. R.; Sniderman, A. D.; Forte, T. M.; Krauss, R. M.; Kwiterovich, P. O.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 80, No. 21 I, 1983, p. 6662-6666.

Research output: Contribution to journalArticle

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AU - Teng, B.

AU - Thompson, G. R.

AU - Sniderman, A. D.

AU - Forte, T. M.

AU - Krauss, R. M.

AU - Kwiterovich, P. O.

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AB - Hyperapobetalipoproteinemia is defined as the combination of a normal low density lipoprotein (LDL) cholesterol in face of an increased LDL apolipoprotein B (apoB) protein. To examine the physical basis for the apparent disproportion between LDL cholesterol and apoB characteristic of this syndrome, we used density gradient ultracentrifugation to isolate LDL fractions from 10 normal subjects, from 20 patients with hyperapobetalipoproteinemia (10 normotriglyceridemic and 10 hypertriglyceridemic), and from 7 patients with familial hypercholesterolemia. In familial hypercholesterolemia, more LDL was in fraction 1 - 'light' LDL - and this LDL was relatively enriched in cholesterol and poor in protein. By contrast, it was fraction 2 - 'heavy' LDL - that differed in hyperapobetalipoproteinemia, being denser, depleted of cholesterol (particularly cholesteryl ester), and relatively enriched in apoB. These findings were more pronounced in the hypertriglyceridemic patients than in the normotriglyceridemic patients with hyperapobetalipoproteinemia. Thus this study confirms that considerable heterogeneity exists between LDL subfractions within individuals but, in addition, indicates there also marked - and apparently characteristic - differences in LDL composition amongst normal subjects and patients with hyperapobetalipoproteinemia or familial hypercholesterolemia.

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