Complex Regulation of the Immunoglobulin μ Heavy-Chain Gene Enhancer: μB, a New Determinant of Enhancer Function

Barbara Nelsen, Tom Kadesch, Ranjan Sen

Research output: Contribution to journalArticle

Abstract

The B-lymphocyte-specific activity of the immunoglobulin μ heavy-chain gene enhancer has been attributed to the octamer motif (ATTTGCAT) present within the enhancer that binds a B-cell-specific factor designated NF-A2/OTF-2. However, significant residual enhancer activity even after deletion of this element has suggested the presence of a second critical functional determinant. We have used deletion and mutational analyses to define an element, μB (TTTGGGGAA), that is essential for B-cell-specific enhancer activity in S194 myeloma cells in the absence of the octamer. Transfection analysis in a panel of lymphoid cell lines suggests that the presence of either μB or octamer leads to considerable enhancer activity in cell lines representing later stages of B-cell differentiation, whereas both elements are needed for function in cell lines representing earlier stages. Furthermore, in contrast to the results in pre-B-cell lines, both μB and octamer elements function independently in certain T-cell lines in which the μ enhancer is active.

Original languageEnglish (US)
Pages (from-to)3145-3154
Number of pages10
JournalMolecular and Cellular Biology
Volume10
Issue number6
StatePublished - Jun 1990
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

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