Complete structure and organization of immunoglobulin heavy chain constant region genes in a phylogenetically primitive vertebrate.

F. Kokubu, K. Hinds, R. Litman, M. J. Shamblott, G. W. Litman

Research output: Contribution to journalArticle

Abstract

Immunoglobulin (Ig) gene organization in Heterodontus francisci (horned shark), a phylogenetically primitive vertebrate, is unique. Homologous Ig heavy chain variable (VH) and constant region (CH) specific probes were used to screen a spleen cDNA library constructed in lambda gt11. Both secretory (SEC) and transmembrane (TM) cDNA clones were recovered; the latter were identified by a negative selection strategy. The complete sequence of the CH portion of a Heterodontus genomic DNA-lambda clone also was determined. The sequences of the individual CH genes differ from each other in all exons. When compared to mammalian prototypes, similarities in exon and intron organization as well as conservation of sequences involved with differential splicing of SEC and TM mRNA indicate that Heterodontus heavy chain genes are of the mu type, although intron lengths are uniformly longer in Heterodontus. Heterodontus genes are not associated, however, with the family of DNA sequences that have been implicated in heavy chain class switching in mammals. Spleen cDNA library screening and RNA blot analyses indicate that mRNAs encoding TM Ig are exceedingly rare. The relationship between this quantitative difference and the distribution of polyadenylation signal sequences suggests that regulation of Ig gene expression in Heterodontus may be highly dependent on position effects.

Original languageEnglish (US)
Pages (from-to)1979-1988
Number of pages10
JournalThe EMBO journal
Volume7
Issue number7
StatePublished - Jul 1988
Externally publishedYes

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Immunoglobulin Heavy Chains
Vertebrates
Immunoglobulin Genes
Genes
Gene Library
Immunoglobulins
Introns
Exons
Spleen
Clone Cells
Immunoglobulin Class Switching
Sharks
Messenger RNA
Polyadenylation
Gene Expression Regulation
Protein Sorting Signals
Mammals
DNA sequences
Complementary DNA
Gene expression

ASJC Scopus subject areas

  • Cell Biology
  • Genetics

Cite this

Complete structure and organization of immunoglobulin heavy chain constant region genes in a phylogenetically primitive vertebrate. / Kokubu, F.; Hinds, K.; Litman, R.; Shamblott, M. J.; Litman, G. W.

In: The EMBO journal, Vol. 7, No. 7, 07.1988, p. 1979-1988.

Research output: Contribution to journalArticle

Kokubu, F, Hinds, K, Litman, R, Shamblott, MJ & Litman, GW 1988, 'Complete structure and organization of immunoglobulin heavy chain constant region genes in a phylogenetically primitive vertebrate.', The EMBO journal, vol. 7, no. 7, pp. 1979-1988.
Kokubu, F. ; Hinds, K. ; Litman, R. ; Shamblott, M. J. ; Litman, G. W. / Complete structure and organization of immunoglobulin heavy chain constant region genes in a phylogenetically primitive vertebrate. In: The EMBO journal. 1988 ; Vol. 7, No. 7. pp. 1979-1988.
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