Complete separation of intracellular and extracellular information in NMR spectra of perfused cells by diffusion-weighted spectroscopy

P. C.M. Van Zijl, C. T.W. Moonen, P. Faustino, J. Pekar, O. Kaplan, J. S. Cohen

Research output: Contribution to journalArticle

Abstract

A method is outlined that completely separates intracellular and extracellular information in NMR spectra of perfused cells. The technique uses diffusion weighting to exploit differences in motional properties between intra- and extracellular constituents. This allows monitoring of intracellular metabolism, and of transport of small drugs and nutrients through the cell membrane, under controlled physiological conditions. As a first example, proton spectra of drug-resistant MCF-7 human breast cancer cells are studied, and uptake of phenylalanine is monitored.

Original languageEnglish (US)
Pages (from-to)3228-3232
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number8
StatePublished - Dec 1 1991
Externally publishedYes

Keywords

  • breast cancer cells
  • intracellular metabolism
  • membrane permeability
  • proton NMR
  • transport

ASJC Scopus subject areas

  • General

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