Complete genomes for hepatitis C virus subtypes 6f, 6i, 6j and 6m: Viral genetic diversity among Thai blood donors and infected spouses

Ling Lu, Chunhua Li, Yongshui Fu, Lakkana Thaikruea, Satawat Thongswat, Niwat Maneekarn, Chatchawann Apichartpiyakul, Hak Hotta, Hiroaki Okamoto, Dale Netski, Oliver G. Pybus, Donald Murphy, Curt H. Hagedorn, Kenrad Edwin Nelson

Research output: Contribution to journalArticle

Abstract

In this study, the first complete genome sequences for hepatitis C virus (HCV) subtypes 6f, 6i, 6j and 6m, obtained from infected blood donors in Chiang Mai, Thailand, are reported. Pairwise genome-wide nucleotide similarities between some of these isolates were higher than the 75-80 % value used previously to define different HCV subtypes. To investigate further, the entire genomes of four prototype isolates, Th602 (6i), Th553 (6j), B4/92 (6m) and D86/93 (6n), were sequenced. Pairwise comparison of these sequences gave a similar range of nucleotide similarities, thereby providing new information for HCV subtype classification. In order to study the hypothesis of interspousal HCV transmission, four additional complete HCV genome sequences were obtained from two infected Thai blood donors and their spouses, C-0044 and C-0046 (6f), and C-0192 and C-0185 (6m). Pairwise comparison of the sequences revealed that C-0044 and C-0046 share a nucleotide similarity of 98.1 %, whilst C-0185 and C-0192 have a similarity of 97.8 %. Several other studies of partial HCV sequences of different genomic regions from HCV-infected couples have shown nucleotide similarities ranging from 96.3 to 100 %. The similarities of the complete genome sequences from the two couples in the current study are consistent with HCV transmission between spouses.

Original languageEnglish (US)
Pages (from-to)1505-1518
Number of pages14
JournalJournal of General Virology
Volume88
Issue number5
DOIs
StatePublished - May 2007

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Blood Donors
Spouses
Hepacivirus
Genome
Nucleotides
Thailand

ASJC Scopus subject areas

  • Virology
  • Immunology

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Complete genomes for hepatitis C virus subtypes 6f, 6i, 6j and 6m : Viral genetic diversity among Thai blood donors and infected spouses. / Lu, Ling; Li, Chunhua; Fu, Yongshui; Thaikruea, Lakkana; Thongswat, Satawat; Maneekarn, Niwat; Apichartpiyakul, Chatchawann; Hotta, Hak; Okamoto, Hiroaki; Netski, Dale; Pybus, Oliver G.; Murphy, Donald; Hagedorn, Curt H.; Nelson, Kenrad Edwin.

In: Journal of General Virology, Vol. 88, No. 5, 05.2007, p. 1505-1518.

Research output: Contribution to journalArticle

Lu, L, Li, C, Fu, Y, Thaikruea, L, Thongswat, S, Maneekarn, N, Apichartpiyakul, C, Hotta, H, Okamoto, H, Netski, D, Pybus, OG, Murphy, D, Hagedorn, CH & Nelson, KE 2007, 'Complete genomes for hepatitis C virus subtypes 6f, 6i, 6j and 6m: Viral genetic diversity among Thai blood donors and infected spouses', Journal of General Virology, vol. 88, no. 5, pp. 1505-1518. https://doi.org/10.1099/vir.0.82604-0
Lu, Ling ; Li, Chunhua ; Fu, Yongshui ; Thaikruea, Lakkana ; Thongswat, Satawat ; Maneekarn, Niwat ; Apichartpiyakul, Chatchawann ; Hotta, Hak ; Okamoto, Hiroaki ; Netski, Dale ; Pybus, Oliver G. ; Murphy, Donald ; Hagedorn, Curt H. ; Nelson, Kenrad Edwin. / Complete genomes for hepatitis C virus subtypes 6f, 6i, 6j and 6m : Viral genetic diversity among Thai blood donors and infected spouses. In: Journal of General Virology. 2007 ; Vol. 88, No. 5. pp. 1505-1518.
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