Complete genome sequence of Salmonella enterica serovar Typhimurium LT2

Michael McClelland, Kenneth E. Sanderson, John Spieth, Sandra W. Clifton, Phil Latreille, Laura Courtney, Steffen Porwollik, Johar Ali, Mike Dante, Feiyu Du, Shunfang Hou, Dan Layman, Shawn Leonard, Christine Nguyen, Kelsi Scott, Andrea Holmes, Neenu Grewal, Elizabeth Mulvaney, Ellen Ryan, Hui SunLiliana Florea, Webb Miller, Tamberlyn Stoneking, Michael Nhan, Robert Waterston, Richard K. Wilson

Research output: Contribution to journalArticle

Abstract

Salmonella enterica subspecies I, serovar Typhimuriurn (S. typhimurium), is a leading cause of human gastroenteritis, and is used as a mouse model of human typhoid fever. The incidence of non-typhoid salmoneliosis is increasing worldwide, causing millions of infections and many deaths in the human population each year. Here we sequenced the 4,857-kilobase (kb) chromosome and 94-kb virulence plasmid of S. typhimurium strain LT2. The distribution of close homologues of S. typhimurium LT2 genes in eight related enterobacteria was determined using previously completed genomes of three related bacteria, sample sequencing of both S. enterica serovar Paratyphi A (S. paratyphi A) and Klebsiella pneumoniae, and hybridization of three unsequenced genomes to a microarray of S. typhimurium LT2 genes. Lateral transfer of genes is frequent, with 11% of the S. typhimurium LT2 genes missing from S. enterica serovar Typhi (S. typhi), and 29% missing from Escherichia coli K12. The 352 gene homologues of S. typhimurium LT2 confined to subspecies I of S. enterica - containing most mammalian and bird pathogens - are useful for studies of epidemiology, host specificity and pathogenesis. Most of these homologues were previously unknown, and 50 may be exported to the periplasm or outer membrane, rendering them accessible as therapeutic or vaccine targets.

Original languageEnglish (US)
Pages (from-to)852-856
Number of pages5
JournalNature
Volume413
Issue number6858
DOIs
StatePublished - Oct 25 2001
Externally publishedYes

ASJC Scopus subject areas

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    McClelland, M., Sanderson, K. E., Spieth, J., Clifton, S. W., Latreille, P., Courtney, L., Porwollik, S., Ali, J., Dante, M., Du, F., Hou, S., Layman, D., Leonard, S., Nguyen, C., Scott, K., Holmes, A., Grewal, N., Mulvaney, E., Ryan, E., ... Wilson, R. K. (2001). Complete genome sequence of Salmonella enterica serovar Typhimurium LT2. Nature, 413(6858), 852-856. https://doi.org/10.1038/35101614