Complete genome sequence of Neisseria meningitidis serogroup B strain MC58

Hervé Tettelin; Nigel J. Saunders; John Heidelberg; Alex C. Jeffries; Karen E. Nelson; Jonathan A. Eisen; Karen A. Ketchum; Derek W. Hood; John F. Peden; Robert J. Dodson; William C. Nelson; Michelle L. Gwinn; Robert DeBoy; Jeremy D. Peterson; Erin K. Hickey; Daniel H. Haft; Steven L. Salzberg; Owen White; Robert D. Fleischmann; Brian A. Dougherty; Tanya Mason; Anne Ciecko; Debbie S. Parksey; Eric Blair; Henry Cittone; Emily B. Clark; Matthew D. Cotton; Terry R. Utterback; Hoda Khouri; Haiying Qin; Jessica Vamathevan; John Gill; Vincenzo Scarlato; Vega Masignani; Mariagrazia Pizza; Guido Grandi; Li Sun; Hamilton O. Smith; Claire M. Fraser; E. Richard Moxon; Rino Rappuoli; J. Craig Venter

doi:10.1126/science.287.5459.1809

Complete genome sequence of Neisseria meningitidis serogroup B strain MC58

Hervé Tettelin, Nigel J. Saunders, John Heidelberg, Alex C. Jeffries, Karen E. Nelson, Jonathan A. Eisen, Karen A. Ketchum, Derek W. Hood, John F. Peden, Robert J. Dodson, William C. Nelson, Michelle L. Gwinn, Robert DeBoy, Jeremy D. Peterson, Erin K. Hickey, Daniel H. Haft, Steven L. Salzberg, Owen White, Robert D. Fleischmann, Brian A. DoughertyTanya Mason, Anne Ciecko, Debbie S. Parksey, Eric Blair, Henry Cittone, Emily B. Clark, Matthew D. Cotton, Terry R. Utterback, Hoda Khouri, Haiying Qin, Jessica Vamathevan, John Gill, Vincenzo Scarlato, Vega Masignani, Mariagrazia Pizza, Guido Grandi, Li Sun, Hamilton O. Smith, Claire M. Fraser, E. Richard Moxon, Rino Rappuoli, J. Craig Venter

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Abstract

The 2,272,351-base pair genome of Neisseria meningitidis strain MC58 (serogroup B), a causative agent of meningitis and septicemia, contains 2158 predicted coding regions, 1158 (53.7%) of which were assigned a biological role. Three major islands of horizontal DNA transfer were identified; two of these contain genes encoding proteins involved in pathogenicity, and the third island contains coding sequences only for hypothetical proteins. Insights into the commensal and virulence behavior of N. meningitidis can be gleaned from the genome, in which sequences for structural proteins of the pilus are clustered and several coding regions unique to serogroup B capsular polysaccharide synthesis can be identified. Finally, N. meningitidis contains more genes that undergo phase variation than any pathogen studied to date, a mechanism that controls their expression and contributes to the evasion of the host immune system.

Original language	English (US)
Pages (from-to)	1809-1815
Number of pages	7
Journal	Science
Volume	287
Issue number	5459
DOIs	https://doi.org/10.1126/science.287.5459.1809
State	Published - Mar 10 2000
Externally published	Yes

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Tettelin, H., Saunders, N. J., Heidelberg, J., Jeffries, A. C., Nelson, K. E., Eisen, J. A., Ketchum, K. A., Hood, D. W., Peden, J. F., Dodson, R. J., Nelson, W. C., Gwinn, M. L., DeBoy, R., Peterson, J. D., Hickey, E. K., Haft, D. H., Salzberg, S. L., White, O., Fleischmann, R. D., ... Craig Venter, J. (2000). Complete genome sequence of Neisseria meningitidis serogroup B strain MC58. Science, 287(5459), 1809-1815. https://doi.org/10.1126/science.287.5459.1809

Tettelin, H, Saunders, NJ, Heidelberg, J, Jeffries, AC, Nelson, KE, Eisen, JA, Ketchum, KA, Hood, DW, Peden, JF, Dodson, RJ, Nelson, WC, Gwinn, ML, DeBoy, R, Peterson, JD, Hickey, EK, Haft, DH, Salzberg, SL, White, O, Fleischmann, RD, Dougherty, BA, Mason, T, Ciecko, A, Parksey, DS, Blair, E, Cittone, H, Clark, EB, Cotton, MD, Utterback, TR, Khouri, H, Qin, H, Vamathevan, J, Gill, J, Scarlato, V, Masignani, V, Pizza, M, Grandi, G, Sun, L, Smith, HO, Fraser, CM, Moxon, ER, Rappuoli, R & Craig Venter, J 2000, 'Complete genome sequence of Neisseria meningitidis serogroup B strain MC58', Science, vol. 287, no. 5459, pp. 1809-1815. https://doi.org/10.1126/science.287.5459.1809

@article{ce078b604c7841248f19a6036d321e7f,

title = "Complete genome sequence of Neisseria meningitidis serogroup B strain MC58",

abstract = "The 2,272,351-base pair genome of Neisseria meningitidis strain MC58 (serogroup B), a causative agent of meningitis and septicemia, contains 2158 predicted coding regions, 1158 (53.7%) of which were assigned a biological role. Three major islands of horizontal DNA transfer were identified; two of these contain genes encoding proteins involved in pathogenicity, and the third island contains coding sequences only for hypothetical proteins. Insights into the commensal and virulence behavior of N. meningitidis can be gleaned from the genome, in which sequences for structural proteins of the pilus are clustered and several coding regions unique to serogroup B capsular polysaccharide synthesis can be identified. Finally, N. meningitidis contains more genes that undergo phase variation than any pathogen studied to date, a mechanism that controls their expression and contributes to the evasion of the host immune system.",

author = "Herv{\'e} Tettelin and Saunders, {Nigel J.} and John Heidelberg and Jeffries, {Alex C.} and Nelson, {Karen E.} and Eisen, {Jonathan A.} and Ketchum, {Karen A.} and Hood, {Derek W.} and Peden, {John F.} and Dodson, {Robert J.} and Nelson, {William C.} and Gwinn, {Michelle L.} and Robert DeBoy and Peterson, {Jeremy D.} and Hickey, {Erin K.} and Haft, {Daniel H.} and Salzberg, {Steven L.} and Owen White and Fleischmann, {Robert D.} and Dougherty, {Brian A.} and Tanya Mason and Anne Ciecko and Parksey, {Debbie S.} and Eric Blair and Henry Cittone and Clark, {Emily B.} and Cotton, {Matthew D.} and Utterback, {Terry R.} and Hoda Khouri and Haiying Qin and Jessica Vamathevan and John Gill and Vincenzo Scarlato and Vega Masignani and Mariagrazia Pizza and Guido Grandi and Li Sun and Smith, {Hamilton O.} and Fraser, {Claire M.} and Moxon, {E. Richard} and Rino Rappuoli and {Craig Venter}, J.",

year = "2000",

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doi = "10.1126/science.287.5459.1809",

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T1 - Complete genome sequence of Neisseria meningitidis serogroup B strain MC58

AU - Tettelin, Hervé

AU - Saunders, Nigel J.

AU - Heidelberg, John

AU - Jeffries, Alex C.

AU - Nelson, Karen E.

AU - Eisen, Jonathan A.

AU - Ketchum, Karen A.

AU - Hood, Derek W.

AU - Peden, John F.

AU - Dodson, Robert J.

AU - Nelson, William C.

AU - Gwinn, Michelle L.

AU - DeBoy, Robert

AU - Peterson, Jeremy D.

AU - Hickey, Erin K.

AU - Haft, Daniel H.

AU - Salzberg, Steven L.

AU - White, Owen

AU - Fleischmann, Robert D.

AU - Dougherty, Brian A.

AU - Mason, Tanya

AU - Ciecko, Anne

AU - Parksey, Debbie S.

AU - Blair, Eric

AU - Cittone, Henry

AU - Clark, Emily B.

AU - Cotton, Matthew D.

AU - Utterback, Terry R.

AU - Khouri, Hoda

AU - Qin, Haiying

AU - Vamathevan, Jessica

AU - Gill, John

AU - Scarlato, Vincenzo

AU - Masignani, Vega

AU - Pizza, Mariagrazia

AU - Grandi, Guido

AU - Sun, Li

AU - Smith, Hamilton O.

AU - Fraser, Claire M.

AU - Moxon, E. Richard

AU - Rappuoli, Rino

AU - Craig Venter, J.

PY - 2000/3/10

Y1 - 2000/3/10

N2 - The 2,272,351-base pair genome of Neisseria meningitidis strain MC58 (serogroup B), a causative agent of meningitis and septicemia, contains 2158 predicted coding regions, 1158 (53.7%) of which were assigned a biological role. Three major islands of horizontal DNA transfer were identified; two of these contain genes encoding proteins involved in pathogenicity, and the third island contains coding sequences only for hypothetical proteins. Insights into the commensal and virulence behavior of N. meningitidis can be gleaned from the genome, in which sequences for structural proteins of the pilus are clustered and several coding regions unique to serogroup B capsular polysaccharide synthesis can be identified. Finally, N. meningitidis contains more genes that undergo phase variation than any pathogen studied to date, a mechanism that controls their expression and contributes to the evasion of the host immune system.

AB - The 2,272,351-base pair genome of Neisseria meningitidis strain MC58 (serogroup B), a causative agent of meningitis and septicemia, contains 2158 predicted coding regions, 1158 (53.7%) of which were assigned a biological role. Three major islands of horizontal DNA transfer were identified; two of these contain genes encoding proteins involved in pathogenicity, and the third island contains coding sequences only for hypothetical proteins. Insights into the commensal and virulence behavior of N. meningitidis can be gleaned from the genome, in which sequences for structural proteins of the pilus are clustered and several coding regions unique to serogroup B capsular polysaccharide synthesis can be identified. Finally, N. meningitidis contains more genes that undergo phase variation than any pathogen studied to date, a mechanism that controls their expression and contributes to the evasion of the host immune system.

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EP - 1815

JO - Science

JF - Science

IS - 5459

ER -

Complete genome sequence of Neisseria meningitidis serogroup B strain MC58

Abstract

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