Complementary roles of specific cysteines in keratin 14 toward the assembly, organization, and dynamics of intermediate filaments in skin keratinocytes

Xia Feng, Pierre A. Coulombe

Research output: Contribution to journalArticle

Abstract

We recently showed that inter-keratin disulfide bonding plays an important role in the assembly, organization, and dynamics of keratin intermediate filaments in skin keratinocytes. In particular, cysteine 367 located in the central α-helical rod domain of keratin 14 is necessary for the formation of a stable perinuclear network of keratin filaments (with type II partner keratin 5) in skin keratinocytes analyzed by static and live cell imaging. Here, we show that two additional cysteine residues located in the non-helical head domain of K14, Cys-4 and Cys-40, also participate in inter-keratin disulfide bonding and tandemly play a key role complementary to that of Cys-367 in the assembly, organization, and dynamics of keratin filaments in skin keratinocytes in primary culture. Analysis of K14 variants with single or multiple substitutions of cysteine residues points to a spatial and temporal hierarchy in how Cys-4/Cys-40 and Cys-367 regulate keratin assembly in vitro and filament dynamics in live keratinocytes in culture. Our findings substantiate the importance and complexity of a novel determinant, namely inter-keratin disulfide bonding, for the regulation of several aspects of keratin filaments in surface epithelia.

Original languageEnglish (US)
Pages (from-to)22507-22519
Number of pages13
JournalJournal of Biological Chemistry
Volume290
Issue number37
DOIs
StatePublished - Sep 11 2015

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Keratin-14
Intermediate Filaments
Keratins
Keratinocytes
Cysteine
Skin
Disulfides
Type II Keratin
Keratin-5
Substitution reactions
Epithelium
Head
Imaging techniques

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Complementary roles of specific cysteines in keratin 14 toward the assembly, organization, and dynamics of intermediate filaments in skin keratinocytes. / Feng, Xia; Coulombe, Pierre A.

In: Journal of Biological Chemistry, Vol. 290, No. 37, 11.09.2015, p. 22507-22519.

Research output: Contribution to journalArticle

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