Complement-Fixing Activity of Rabbit Antibody Reconstructed from Half Molecules

Kimishige Ishizaka, Teruko Ishizaka, Jay Banovitz

Research output: Contribution to journalArticle

Abstract

Rabbit anti-BSA antibody was reduced in 0.1 M mercaptoethylamine (pH 5.0) followed by alkylation with iodoacetate. Specific precipitates formed with the treated antibody and antigen fixed C. The C′-fixing activity of the specific precipitates did not change by the treatment at pH 2.5 in 0.1 M NaCl, which results in dissociation of the precipitates into BSA and half molecules of antibody. The results indicate that the antibody reconstructed from the half molecules fixes C with antigen and that inter-H chain disulfide linkages in original antibody molecules are not essential for C fixation.

Original languageEnglish (US)
Pages (from-to)507-511
Number of pages5
JournalProceedings of the Society for Experimental Biology and Medicine
Volume118
Issue number2
DOIs
StatePublished - Feb 1965

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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