Compensation-free, all-fiber-optic, two-photon endomicroscopy at 1.55 μm

Kartikeya Murari, Yuying Zhang, Shenping Li, Yongping Chen, Ming Jun Li, Xingde Li

Research output: Contribution to journalArticle

Abstract

We present an all-fiber-optic scanning multiphoton endomicroscope with 1.55 μm excitation without the need for prechirping femtosecond pulses before the endomicroscope. The system consists of a 1.55 μm femtosecond fiber laser, a customized double-clad fiber for light delivery and fluorescence collection, and a piezoelectric scan head. We demonstrate two-photon imaging of cultured cells and mouse tissue, both labeled with indocyanine green. Free-space multiphoton imaging with near-IR emission has previously shown benefits in reduced background fluorescence and lower attenuation for the fluorescence emission. For fiber-optic multiphoton imaging there is the additional advantage of using the soliton effect at the telecommunication wavelengths (1.3-1.6 μm) in fibers, permitting dispersion-compensation-free, small-footprint systems. We expect these advantages will help transition multiphoton endomicroscopy to the clinic.

Original languageEnglish (US)
Pages (from-to)1299-1301
Number of pages3
JournalOptics Letters
Volume36
Issue number7
DOIs
StatePublished - Apr 1 2011

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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