Comparisons of alloreactive potential of clinical hematopoietic grafts

Wing Leung, Manuel Ramírez, Goutam Mukherjee, Elizabeth J. Perlman, Curt I. Civin

Research output: Contribution to journalArticle

Abstract

Background. We sought to compare the immunoreactive potential of human cord blood (CB) versus normal adult bone marrow (BM) versus mobilized blood (peripheral blood stem cells; PBSC) from cancer patients. Methods. Forty mice were randomized to receive a range of doses of T cell-replete cell preparations from one of the above three cell sources. Twenty-eight control mice underwent transplantation with T cell-depleted cells. Mice were observed for 60 days for the development of fatal xenogeneic graft-versus-host disease-like syndrome (GVHDLS). Results. For the mice that had received T cell-replete grafts of CB or BM or PBSC, the duration of GVHDLS free survival of the chimeras was inversely proportional to the number of T cells transplanted. After adjustment for the number of T cells transplanted, the relative hazard of developing fatal GVHDLS was 62-fold higher for PBSC and 210-fold higher for BM as compared with CB. Flow cytometric and histologic analyses of selected chimeras that died of GVHDLS showed extensive proliferation of human T cells in multiple organs. In contrast, mice that survived to day 60 were engrafted with human myeloid and B lymphoid cells. Conclusions. The immunoreactive potential, as measured by this in vivo assay, differed among clinical grafts: BM > PBSC > CB.

Original languageEnglish (US)
Pages (from-to)628-635
Number of pages8
JournalTransplantation
Volume68
Issue number5
DOIs
StatePublished - Sep 15 1999

ASJC Scopus subject areas

  • Transplantation
  • Immunology

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    Leung, W., Ramírez, M., Mukherjee, G., Perlman, E. J., & Civin, C. I. (1999). Comparisons of alloreactive potential of clinical hematopoietic grafts. Transplantation, 68(5), 628-635. https://doi.org/10.1097/00007890-199909150-00006