Comparison of two toxins produced by Clostridium difficile

N. S. Taylor, G. M. Thorne, John Bartlett

Research output: Contribution to journalArticle

Abstract

Clostridium difficile was shown to produce A toxin which could be biochemically separated from the previously described cytotoxin of the same organism. The two proteins differ in biological activity and physical properties. Antiserum prepared to the second toxin does not neutralize the biological activity of the cytotoxin, and immunological cross-reactivity could not be demonstrated. However, some relationship may exist between the two toxins, since the newly described toxin degrades on polyacrylamide electrophoresis into two molecules, one of which appears to migrate with the band of purified cytotoxin. We suggest that this newly described toxin be designated toxin A until its primary biological activity and physical relationship to cytotoxin is determined. This toxin is active in biological assays of enteric disease and may play an important role in C. difficile-induced colitis.

Original languageEnglish (US)
Pages (from-to)1036-1043
Number of pages8
JournalInfection and Immunity
Volume34
Issue number3
StatePublished - 1981
Externally publishedYes

Fingerprint

Clostridium difficile
Cytotoxins
Colitis
Biological Assay
Electrophoresis
Immune Sera
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Comparison of two toxins produced by Clostridium difficile. / Taylor, N. S.; Thorne, G. M.; Bartlett, John.

In: Infection and Immunity, Vol. 34, No. 3, 1981, p. 1036-1043.

Research output: Contribution to journalArticle

Taylor, NS, Thorne, GM & Bartlett, J 1981, 'Comparison of two toxins produced by Clostridium difficile', Infection and Immunity, vol. 34, no. 3, pp. 1036-1043.
Taylor, N. S. ; Thorne, G. M. ; Bartlett, John. / Comparison of two toxins produced by Clostridium difficile. In: Infection and Immunity. 1981 ; Vol. 34, No. 3. pp. 1036-1043.
@article{ddb8a53319f9405f837c95c3a5277e64,
title = "Comparison of two toxins produced by Clostridium difficile",
abstract = "Clostridium difficile was shown to produce A toxin which could be biochemically separated from the previously described cytotoxin of the same organism. The two proteins differ in biological activity and physical properties. Antiserum prepared to the second toxin does not neutralize the biological activity of the cytotoxin, and immunological cross-reactivity could not be demonstrated. However, some relationship may exist between the two toxins, since the newly described toxin degrades on polyacrylamide electrophoresis into two molecules, one of which appears to migrate with the band of purified cytotoxin. We suggest that this newly described toxin be designated toxin A until its primary biological activity and physical relationship to cytotoxin is determined. This toxin is active in biological assays of enteric disease and may play an important role in C. difficile-induced colitis.",
author = "Taylor, {N. S.} and Thorne, {G. M.} and John Bartlett",
year = "1981",
language = "English (US)",
volume = "34",
pages = "1036--1043",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "3",

}

TY - JOUR

T1 - Comparison of two toxins produced by Clostridium difficile

AU - Taylor, N. S.

AU - Thorne, G. M.

AU - Bartlett, John

PY - 1981

Y1 - 1981

N2 - Clostridium difficile was shown to produce A toxin which could be biochemically separated from the previously described cytotoxin of the same organism. The two proteins differ in biological activity and physical properties. Antiserum prepared to the second toxin does not neutralize the biological activity of the cytotoxin, and immunological cross-reactivity could not be demonstrated. However, some relationship may exist between the two toxins, since the newly described toxin degrades on polyacrylamide electrophoresis into two molecules, one of which appears to migrate with the band of purified cytotoxin. We suggest that this newly described toxin be designated toxin A until its primary biological activity and physical relationship to cytotoxin is determined. This toxin is active in biological assays of enteric disease and may play an important role in C. difficile-induced colitis.

AB - Clostridium difficile was shown to produce A toxin which could be biochemically separated from the previously described cytotoxin of the same organism. The two proteins differ in biological activity and physical properties. Antiserum prepared to the second toxin does not neutralize the biological activity of the cytotoxin, and immunological cross-reactivity could not be demonstrated. However, some relationship may exist between the two toxins, since the newly described toxin degrades on polyacrylamide electrophoresis into two molecules, one of which appears to migrate with the band of purified cytotoxin. We suggest that this newly described toxin be designated toxin A until its primary biological activity and physical relationship to cytotoxin is determined. This toxin is active in biological assays of enteric disease and may play an important role in C. difficile-induced colitis.

UR - http://www.scopus.com/inward/record.url?scp=0019859594&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019859594&partnerID=8YFLogxK

M3 - Article

C2 - 7333662

AN - SCOPUS:0019859594

VL - 34

SP - 1036

EP - 1043

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 3

ER -