Comparison of three methodologies for the determination of pulmonary fungal burden in experimental murine aspergillosis

Donald C. Sheppard; K. A. Marr; D. N. Fredricks; L. Y. Chiang; T. Doedt; S. G. Filler

doi:10.1111/j.1469-0691.2005.01349.x

Comparison of three methodologies for the determination of pulmonary fungal burden in experimental murine aspergillosis

Donald C. Sheppard, K. A. Marr, D. N. Fredricks, L. Y. Chiang, T. Doedt, S. G. Filler

Research output: Contribution to journal › Article › peer-review

52 Scopus citations

Abstract

Quantitative culture, quantitative PCR and the galactomannan enzyme immunoassay (EIA) were compared for their ability to determine the pulmonary fungal burden in a murine model of invasive aspergillosis. Quantitative culture of specimens containing hyphae under-represented the absolute fungal burden in established infection when compared with the two other methods. The best correlation was observed between the two non-culture methods. Higher variability was observed with the galactomannan EIA when compared with quantitative PCR. Collectively, these data suggest that quantitative PCR is the preferred method for determination of the pulmonary fungal burden in experimental aspergillosis.

Original language	English (US)
Pages (from-to)	376-380
Number of pages	5
Journal	Clinical Microbiology and Infection
Volume	12
Issue number	4
DOIs	https://doi.org/10.1111/j.1469-0691.2005.01349.x
State	Published - Apr 2006
Externally published	Yes

Keywords

Aspergillosis
Fungal burden
Galactomannan
Murine model
PCR
Quantitative culture

ASJC Scopus subject areas

Microbiology (medical)
Infectious Diseases

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10.1111/j.1469-0691.2005.01349.x

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title = "Comparison of three methodologies for the determination of pulmonary fungal burden in experimental murine aspergillosis",

abstract = "Quantitative culture, quantitative PCR and the galactomannan enzyme immunoassay (EIA) were compared for their ability to determine the pulmonary fungal burden in a murine model of invasive aspergillosis. Quantitative culture of specimens containing hyphae under-represented the absolute fungal burden in established infection when compared with the two other methods. The best correlation was observed between the two non-culture methods. Higher variability was observed with the galactomannan EIA when compared with quantitative PCR. Collectively, these data suggest that quantitative PCR is the preferred method for determination of the pulmonary fungal burden in experimental aspergillosis.",

keywords = "Aspergillosis, Fungal burden, Galactomannan, Murine model, PCR, Quantitative culture",

author = "Sheppard, {Donald C.} and Marr, {K. A.} and Fredricks, {D. N.} and Chiang, {L. Y.} and T. Doedt and Filler, {S. G.}",

note = "Funding Information: DCS is supported by a Burroughs Welcome Fund Career Award in the Biomedical Sciences and a Clinician Scientist award from the Canadian Institutes of Health. This project was supported, in part, with federal funds from the National Institute of Allergy and Infectious Diseases (contract numbers N01-AI-30041 1 and U01 AI-054736). The Platelia Galactomannan EIA kits were provided by Bio-Rad Laboratories (Hercules, CA, USA). We thank C. Smith for her technical contributions.",

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doi = "10.1111/j.1469-0691.2005.01349.x",

language = "English (US)",

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AU - Sheppard, Donald C.

AU - Marr, K. A.

AU - Fredricks, D. N.

AU - Chiang, L. Y.

AU - Doedt, T.

AU - Filler, S. G.

N1 - Funding Information: DCS is supported by a Burroughs Welcome Fund Career Award in the Biomedical Sciences and a Clinician Scientist award from the Canadian Institutes of Health. This project was supported, in part, with federal funds from the National Institute of Allergy and Infectious Diseases (contract numbers N01-AI-30041 1 and U01 AI-054736). The Platelia Galactomannan EIA kits were provided by Bio-Rad Laboratories (Hercules, CA, USA). We thank C. Smith for her technical contributions.

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N2 - Quantitative culture, quantitative PCR and the galactomannan enzyme immunoassay (EIA) were compared for their ability to determine the pulmonary fungal burden in a murine model of invasive aspergillosis. Quantitative culture of specimens containing hyphae under-represented the absolute fungal burden in established infection when compared with the two other methods. The best correlation was observed between the two non-culture methods. Higher variability was observed with the galactomannan EIA when compared with quantitative PCR. Collectively, these data suggest that quantitative PCR is the preferred method for determination of the pulmonary fungal burden in experimental aspergillosis.

AB - Quantitative culture, quantitative PCR and the galactomannan enzyme immunoassay (EIA) were compared for their ability to determine the pulmonary fungal burden in a murine model of invasive aspergillosis. Quantitative culture of specimens containing hyphae under-represented the absolute fungal burden in established infection when compared with the two other methods. The best correlation was observed between the two non-culture methods. Higher variability was observed with the galactomannan EIA when compared with quantitative PCR. Collectively, these data suggest that quantitative PCR is the preferred method for determination of the pulmonary fungal burden in experimental aspergillosis.

KW - Aspergillosis

KW - Fungal burden

KW - Galactomannan

KW - Murine model

KW - PCR

KW - Quantitative culture

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Comparison of three methodologies for the determination of pulmonary fungal burden in experimental murine aspergillosis

Abstract

Keywords

ASJC Scopus subject areas

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