Comparison of three methodologies for the determination of pulmonary fungal burden in experimental murine aspergillosis

Donald C. Sheppard, K. A. Marr, D. N. Fredricks, L. Y. Chiang, T. Doedt, S. G. Filler

Research output: Contribution to journalArticlepeer-review

Abstract

Quantitative culture, quantitative PCR and the galactomannan enzyme immunoassay (EIA) were compared for their ability to determine the pulmonary fungal burden in a murine model of invasive aspergillosis. Quantitative culture of specimens containing hyphae under-represented the absolute fungal burden in established infection when compared with the two other methods. The best correlation was observed between the two non-culture methods. Higher variability was observed with the galactomannan EIA when compared with quantitative PCR. Collectively, these data suggest that quantitative PCR is the preferred method for determination of the pulmonary fungal burden in experimental aspergillosis.

Original languageEnglish (US)
Pages (from-to)376-380
Number of pages5
JournalClinical Microbiology and Infection
Volume12
Issue number4
DOIs
StatePublished - Apr 2006
Externally publishedYes

Keywords

  • Aspergillosis
  • Fungal burden
  • Galactomannan
  • Murine model
  • PCR
  • Quantitative culture

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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