Comparison of three commercial immunoassays for detection of herpes simplex virus type 2 antibodies in commercial sex workers in Yunnan Province, China

Thoai D. Ngo, Oliver B. Laeyendecker, Rhoda Ashley Morrow, Shenghan Lai, Thomas C Quinn

Research output: Contribution to journalArticle

Abstract

Five hundred commercial sex workers in China were tested for herpes simplex virus type 2 by three immunoassays and Western blotting. Sensitivities for the Focus, Kalon, and Biokit assays were 86.7%, 82.3%, and 34.9%, respectively, and specificities were 91.8%, 94.2%, and 60.1%, respectively. The Focus assay performed optimally at an index of 1.5 (95.2% sensitivity and 93.4% specificity), and the Kalon assay performed optimally at an index of 1.2 (93.3% sensitivity and 95.2% specificity).

Original languageEnglish (US)
Pages (from-to)1301-1303
Number of pages3
JournalClinical and Vaccine Immunology
Volume15
Issue number8
DOIs
StatePublished - Aug 2008

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Sex Workers
Human Herpesvirus 2
Viruses
Immunoassay
Assays
China
Sensitivity and Specificity
Antibodies
Western Blotting

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Immunology
  • Immunology and Allergy
  • Microbiology (medical)

Cite this

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title = "Comparison of three commercial immunoassays for detection of herpes simplex virus type 2 antibodies in commercial sex workers in Yunnan Province, China",
abstract = "Five hundred commercial sex workers in China were tested for herpes simplex virus type 2 by three immunoassays and Western blotting. Sensitivities for the Focus, Kalon, and Biokit assays were 86.7{\%}, 82.3{\%}, and 34.9{\%}, respectively, and specificities were 91.8{\%}, 94.2{\%}, and 60.1{\%}, respectively. The Focus assay performed optimally at an index of 1.5 (95.2{\%} sensitivity and 93.4{\%} specificity), and the Kalon assay performed optimally at an index of 1.2 (93.3{\%} sensitivity and 95.2{\%} specificity).",
author = "Ngo, {Thoai D.} and Laeyendecker, {Oliver B.} and Morrow, {Rhoda Ashley} and Shenghan Lai and Quinn, {Thomas C}",
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AU - Lai, Shenghan

AU - Quinn, Thomas C

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AB - Five hundred commercial sex workers in China were tested for herpes simplex virus type 2 by three immunoassays and Western blotting. Sensitivities for the Focus, Kalon, and Biokit assays were 86.7%, 82.3%, and 34.9%, respectively, and specificities were 91.8%, 94.2%, and 60.1%, respectively. The Focus assay performed optimally at an index of 1.5 (95.2% sensitivity and 93.4% specificity), and the Kalon assay performed optimally at an index of 1.2 (93.3% sensitivity and 95.2% specificity).

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