Comparison of the sulforhodamine B protein and tetrazolium (MTT) assays for in vitro chemosensitivity testing

Yvonne P. Keepers, Paulo E. Pizao, Godefridus J. Peters, Jannette van Ark-Otte, Benjamin Winograd, Herbert M. Pinedo

Research output: Contribution to journalArticle

Abstract

The sulforhodamine B (SRB) protein stain assay was compared with the tetrazolium (MTT) colorimetric assay for in vitro chemosensitivity testing of various human tumour cell lines. The SRB assay provided a better linearity with cell number and a higher sensitivity, and its staining was not cell-line dependent. In contrast to the MTT assay, the SRB assay stained recently lysed cells. Cell debris, however, was not stained by SRB and therefore the drug sensitivity data were not affected.

Original languageEnglish (US)
Pages (from-to)897-900
Number of pages4
JournalEuropean Journal of Cancer and Clinical Oncology
Volume27
Issue number7
DOIs
StatePublished - 1991
Externally publishedYes

Fingerprint

lissamine rhodamine B
Proteins
Tumor Cell Line
Coloring Agents
Cell Count
Staining and Labeling
Cell Line
thiazolyl blue
In Vitro Techniques
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Comparison of the sulforhodamine B protein and tetrazolium (MTT) assays for in vitro chemosensitivity testing. / Keepers, Yvonne P.; Pizao, Paulo E.; Peters, Godefridus J.; van Ark-Otte, Jannette; Winograd, Benjamin; Pinedo, Herbert M.

In: European Journal of Cancer and Clinical Oncology, Vol. 27, No. 7, 1991, p. 897-900.

Research output: Contribution to journalArticle

Keepers, Yvonne P. ; Pizao, Paulo E. ; Peters, Godefridus J. ; van Ark-Otte, Jannette ; Winograd, Benjamin ; Pinedo, Herbert M. / Comparison of the sulforhodamine B protein and tetrazolium (MTT) assays for in vitro chemosensitivity testing. In: European Journal of Cancer and Clinical Oncology. 1991 ; Vol. 27, No. 7. pp. 897-900.
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AU - Pinedo, Herbert M.

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