TY - JOUR
T1 - Comparison of the sulforhodamine B protein and tetrazolium (MTT) assays for in vitro chemosensitivity testing
AU - Keepers, Yvonne P.
AU - Pizao, Paulo E.
AU - Peters, Godefridus J.
AU - van Ark-Otte, Jannette
AU - Winograd, Benjamin
AU - Pinedo, Herbert M.
PY - 1991
Y1 - 1991
N2 - The sulforhodamine B (SRB) protein stain assay was compared with the tetrazolium (MTT) colorimetric assay for in vitro chemosensitivity testing of various human tumour cell lines. The SRB assay provided a better linearity with cell number and a higher sensitivity, and its staining was not cell-line dependent. In contrast to the MTT assay, the SRB assay stained recently lysed cells. Cell debris, however, was not stained by SRB and therefore the drug sensitivity data were not affected.
AB - The sulforhodamine B (SRB) protein stain assay was compared with the tetrazolium (MTT) colorimetric assay for in vitro chemosensitivity testing of various human tumour cell lines. The SRB assay provided a better linearity with cell number and a higher sensitivity, and its staining was not cell-line dependent. In contrast to the MTT assay, the SRB assay stained recently lysed cells. Cell debris, however, was not stained by SRB and therefore the drug sensitivity data were not affected.
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U2 - 10.1016/0277-5379(91)90142-Z
DO - 10.1016/0277-5379(91)90142-Z
M3 - Article
C2 - 1834124
AN - SCOPUS:0025878872
SN - 0277-5379
VL - 27
SP - 897
EP - 900
JO - European Journal of Cancer and Clinical Oncology
JF - European Journal of Cancer and Clinical Oncology
IS - 7
ER -