Comparison of the reactivity of nitric oxide and nitroxyl with heme proteins

A chemical discussion of the differential biological effects of these redox related products of NOS

Katrina M. Miranda, Raymond W. Nims, Douglas D. Thomas, Michael G. Espey, Deborah Citrin, Michael D. Bartberger, Nazareno Paolocci, Jon M. Fukuto, Martin Feelisch, David A. Wink

Research output: Contribution to journalArticle

Abstract

Investigations on the biological effects of nitric oxide (NO) derived from nitric oxide synthase (NOS) have led to an explosion in biomedical research over the last decade. The chemistry of this diatomic radical is key to its biological effects. Recently, nitroxyl (HNO/NO-) has been proposed to be another important constituent of NO biology. However, these redox siblings often exhibit orthogonal behavior in physiological and cellular responses. We therefore explored the chemistry of NO and HNO with heme proteins in different redox states and observed that HNO favors reaction with ferric heme while NO favors ferrous, consistent with previous reports. Further results show that HNO and NO were equally effective in inhibiting cytochrome P450 activity, which involves ferric and ferrous complexes. The differential chemical behavior of NO and HNO toward heme proteins provides insight into mechanisms of activity that not only helps explain some of the opposing effects observed in NOS-mediated events, but offers a unique control mechanism for the biological action of NO.

Original languageEnglish (US)
Pages (from-to)52-60
Number of pages9
JournalJournal of Inorganic Biochemistry
Volume93
Issue number1-2
DOIs
StatePublished - Jan 1 2003

Fingerprint

Hemeproteins
Staphylococcal Protein A
Nitric Oxide Synthase
Oxidation-Reduction
Nitric Oxide
nitroxyl
Explosions
Heme
Cytochrome P-450 Enzyme System
Biomedical Research

Keywords

  • Angeli's salt
  • Heme
  • Hemoglobin
  • Horseradish peroxidase
  • Myoglobin
  • Nitric oxide
  • Nitroxyl

ASJC Scopus subject areas

  • Biochemistry
  • Inorganic Chemistry

Cite this

Comparison of the reactivity of nitric oxide and nitroxyl with heme proteins : A chemical discussion of the differential biological effects of these redox related products of NOS. / Miranda, Katrina M.; Nims, Raymond W.; Thomas, Douglas D.; Espey, Michael G.; Citrin, Deborah; Bartberger, Michael D.; Paolocci, Nazareno; Fukuto, Jon M.; Feelisch, Martin; Wink, David A.

In: Journal of Inorganic Biochemistry, Vol. 93, No. 1-2, 01.01.2003, p. 52-60.

Research output: Contribution to journalArticle

Miranda, Katrina M. ; Nims, Raymond W. ; Thomas, Douglas D. ; Espey, Michael G. ; Citrin, Deborah ; Bartberger, Michael D. ; Paolocci, Nazareno ; Fukuto, Jon M. ; Feelisch, Martin ; Wink, David A. / Comparison of the reactivity of nitric oxide and nitroxyl with heme proteins : A chemical discussion of the differential biological effects of these redox related products of NOS. In: Journal of Inorganic Biochemistry. 2003 ; Vol. 93, No. 1-2. pp. 52-60.
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