Comparison of the reactivity of human and rabbit blood towards pyrogenic stimuli.

Stefanie Schindler; Adrian Bristow; Tammy Cartmell; Thomas Hartung; Stefan Fennrich

Comparison of the reactivity of human and rabbit blood towards pyrogenic stimuli.

Stefanie Schindler, Adrian Bristow, Tammy Cartmell, Thomas Hartung, Stefan Fennrich

Research output: Contribution to journal › Article › peer-review

Abstract

A comparison between humans and rabbits was performed based on stimulation of whole blood with well-known pyrogens from Gram-negative and Gram-positive bacteria, such as lipopolysaccharide (LPS) and lipoteichoic acid (LTA), respectively. The reactivity was measured as release of IL-1 beta and IL-8 by ELISA. The reactivity of the two species towards LPS was found to be similar, whereas their reactivity towards LTA differed considerably. Differences between the levels of IL-1 beta and IL-8 release were observed in both species. This finding suggests that the In vitro Pyrogen Test (IPT) which uses human blood to detect contaminations, e.g. of injectable drugs, might predict the human reaction to the contamination better than the "gold standard" rabbit pyrogen test.

Original language	English (US)
Pages (from-to)	59-63
Number of pages	5
Journal	ALTEX : Alternativen zu Tierexperimenten
Volume	20
Issue number	2
State	Published - 2003
Externally published	Yes

ASJC Scopus subject areas

Pharmacology
Medical Laboratory Technology

Cite this

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AU - Fennrich, Stefan

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Y1 - 2003

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AB - A comparison between humans and rabbits was performed based on stimulation of whole blood with well-known pyrogens from Gram-negative and Gram-positive bacteria, such as lipopolysaccharide (LPS) and lipoteichoic acid (LTA), respectively. The reactivity was measured as release of IL-1 beta and IL-8 by ELISA. The reactivity of the two species towards LPS was found to be similar, whereas their reactivity towards LTA differed considerably. Differences between the levels of IL-1 beta and IL-8 release were observed in both species. This finding suggests that the In vitro Pyrogen Test (IPT) which uses human blood to detect contaminations, e.g. of injectable drugs, might predict the human reaction to the contamination better than the "gold standard" rabbit pyrogen test.

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Comparison of the reactivity of human and rabbit blood towards pyrogenic stimuli.

Abstract

ASJC Scopus subject areas

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