Comparison of the Phadebact gonoccocus test with the rapid fermentation method

S. L. Futrovsky, C. A. Gaydos, J. Keiser

Research output: Contribution to journalArticle

Abstract

The Phadebact Gonococcus Test (Pharmacia Diagnostics, Piscataway, N.J.), a coagglutination technique, was compared with the rapid fermentation method of Kellogg and Turner (D.S. Kellogg, Jr., and E.M. Turner, Appl. Microbiol. 25: 550-552, 1973). A total of 93 organisms isolated on Martin-Lewis media were determined to be Neisseria gonorrhoeae based on the following criteria: presence of gram-negative diplococci, oxidase positivity, and appropriate reaction in the rapid fermentation method. These 93 isolates were then serologically tested with the Phadebact test. The direct method was attempted on the first 46 N. gonorrhoeae isolates. Difficulty in interpreting results was encountered in 39%. Thereafter, the alternate method of boiling was instituted on an additional 47 N. gonorrhoeae isolates, with 2 isolates producing noninterpretable results. All 93 isolates were frozen for a maximum of 2 months in skim milk at -25°C. These isolates were thawed and retyped with the alternate boiling procedure, with 97% being confirmed as N. gonorrhoeae. In addition, 33 Neisseria meningitidis isolates, 14 Neisseria species, and 7 Moraxella species were tested with similar techniques. No positive reactions were observed. A cost effectiveness study using 5, 10, and 20 μl of the gonoccocal reagent was undertaken to reduce the cost of the test. When 10 and 20 μl of reagent were used, no difficulty was encountered in interpreting the reaction. The coagglutination technique was difficult to read when 5 μl of reagent was used.

Original languageEnglish (US)
Pages (from-to)89-93
Number of pages5
JournalJournal of clinical microbiology
Volume14
Issue number1
DOIs
StatePublished - Jan 1 1981

ASJC Scopus subject areas

  • Microbiology (medical)

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