Comparison of the peptidase activity in the oncosphere excretory/secretory products of Taenia solium and Taenia saginata

Mirko Zimic, Jesús Infantes, César López, Jeanette Velásquez, Marilú Farfán, Mónica Pajuelo, Patricia Sheen, Manuela Verastegui, Armandoe Gonzalez, Hector H. García, Robert H Gilman

Research output: Contribution to journalArticle

Abstract

We compared the peptidase activities of the excretory/secretory (E/S) antigens of oncospheres of Taenia solium and related, but nonpathogenic, Taenia saginata. Taenia solium and T. saginata oncospheres were cultured, and the spent media of 24-, 48-, 72-, and 96-hr fractions were analyzed. Activities for serine peptidases (chymotrypsin-, trypsin-, and elastase-like), cysteine peptidases (cathepsin B-, cathepsin L-, and calpaine-like), and aminopeptidase (B-like peptidases) were tested fluorometrically with peptides coupled to 7-amino-4-methylcoumarin. In both species, the E/S antigens showed cysteine, serine, and aminopeptidase activities. Although no particular peptidase had high activity in T. solium, and was absent in T. saginata, or vice versa, different patterns of activity were found. A chymotrypsin-like peptidase showed the highest activity in both parasites, and it had 10 times higher activity in T. solium than in T. saginata. Trypsin-like and cathepsin B-like activities were significantly higher in T. solium. Minimal levels of cathepsin B were present in both species, and higher levels of elastase-like and cathepsin L-like activity were observed in T. saginata. Taenia solium and T. saginata have different levels and temporal activities of proteolytic enzymes that could play a modulator role in the host specificity for larval invasion through penetration of the intestinal mucosa.

Original languageEnglish (US)
Pages (from-to)727-734
Number of pages8
JournalJournal of Parasitology
Volume93
Issue number4
DOIs
StatePublished - Aug 2007

Fingerprint

Taenia saginata
oncospheres
Taenia solium
excretory-secretory products
peptidases
antigen
Peptide Hydrolases
cathepsin B
Cathepsin B
host specificity
peptide
cathepsin L
Cathepsin L
elastase
parasite
penetration
aminopeptidases
Pancreatic Elastase
tripeptide aminopeptidase
chymotrypsin

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Parasitology
  • Microbiology

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Comparison of the peptidase activity in the oncosphere excretory/secretory products of Taenia solium and Taenia saginata. / Zimic, Mirko; Infantes, Jesús; López, César; Velásquez, Jeanette; Farfán, Marilú; Pajuelo, Mónica; Sheen, Patricia; Verastegui, Manuela; Gonzalez, Armandoe; García, Hector H.; Gilman, Robert H.

In: Journal of Parasitology, Vol. 93, No. 4, 08.2007, p. 727-734.

Research output: Contribution to journalArticle

Zimic, M, Infantes, J, López, C, Velásquez, J, Farfán, M, Pajuelo, M, Sheen, P, Verastegui, M, Gonzalez, A, García, HH & Gilman, RH 2007, 'Comparison of the peptidase activity in the oncosphere excretory/secretory products of Taenia solium and Taenia saginata', Journal of Parasitology, vol. 93, no. 4, pp. 727-734. https://doi.org/10.1645/GE-959R.1
Zimic, Mirko ; Infantes, Jesús ; López, César ; Velásquez, Jeanette ; Farfán, Marilú ; Pajuelo, Mónica ; Sheen, Patricia ; Verastegui, Manuela ; Gonzalez, Armandoe ; García, Hector H. ; Gilman, Robert H. / Comparison of the peptidase activity in the oncosphere excretory/secretory products of Taenia solium and Taenia saginata. In: Journal of Parasitology. 2007 ; Vol. 93, No. 4. pp. 727-734.
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AU - Velásquez, Jeanette

AU - Farfán, Marilú

AU - Pajuelo, Mónica

AU - Sheen, Patricia

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