Comparison of the nucleotide sequences of 16S rRNA, 444 Ep-ank, and groESL heat shock operon genes in naturally occurring Ehrlichia equi and human granulocytic Ehrlichiosis agent isolates from northern California

Joon Seok Chae, Janet E. Foley, J. Stephen Dumler, John E. Madigan

Research output: Contribution to journalArticle

Abstract

We examined 11 naturally occurring isolates of Ehrlichia equi in horses and two human granulocytic ehrlichiosis agent isolates in California for sequence diversity in three genes. Ehrlichia equi isolates were from Sierra (n = 6), Mendocino (n = 3), Sonoma (n = 1), and Marin (n = 1) counties, and human granulocytic ehrlichiosis (HGE) agent isolates were obtained from Humboldt county. PCR with specific primers for 16S rRNA, 444 Ep-ank and groESL heat shock operon genes successfully produced amplicons for all 13 clinical samples. The 444 Ep-ank gene of the HGE agent and E. equi isolates from northern California is different from the eastern U.S. isolates BDS and USG3. The translated amino acid sequence of the groESL heat shock operon gene fragment is identical among E. equi, the HGE agent, and E. phagocytophila, with the exception of the northern Californian equine CASOLJ isolate. Microheterogeneity was observed in the 16S rRNA gene sequences of HGE agent and E. equi isolates from northern California. These results suggest that E. equi and the HGE agent found in California are similar or identical but may differ from the isolates of equine and human origin found in the eastern United States.

Original languageEnglish (US)
Pages (from-to)1364-1369
Number of pages6
JournalJournal of Clinical Microbiology
Volume38
Issue number4
StatePublished - 2000

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Anaplasma phagocytophilum
Ehrlichiosis
Operon
Shock
Hot Temperature
Genes
Horses
rRNA Genes
Amino Acid Sequence
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

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Comparison of the nucleotide sequences of 16S rRNA, 444 Ep-ank, and groESL heat shock operon genes in naturally occurring Ehrlichia equi and human granulocytic Ehrlichiosis agent isolates from northern California. / Chae, Joon Seok; Foley, Janet E.; Dumler, J. Stephen; Madigan, John E.

In: Journal of Clinical Microbiology, Vol. 38, No. 4, 2000, p. 1364-1369.

Research output: Contribution to journalArticle

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