Comparison of the antibodies in lymphocyte supernatant and antibody-secreting cell assays for measuring intestinal mucosal immune response to a novel oral typhoid vaccine (M01ZH09)

B. D. Kirkpatrick, Matthew D. Bentley, Anette M. Thern, Catherine J. Larsson, Cassandra Ventrone, Meera V. Sreenivasan, Lou Bourgeois

Research output: Contribution to journalArticlepeer-review

Abstract

Antibody-secreting cell (ASC) and antibodies in lymphocyte supernatant (ALS) assays are used to assess intestinal mucosal responses to enteric infections and vaccines. The ALS assay, performed on cell supernatants, may represent a convenient alternative to the more established ASC assay. The two methods, measuring immunoglobulin A to Salmonella enterica serovar Typhi lipopolysaccharide, were compared in volunteers vaccinated with a live-attenuated typhoid vaccine M01ZH09. The specificity of the ALS assay compared to the ASC assay was excellent (100%), as was sensitivity (82%). The ALS assay was less sensitive than the ASC assay at ≤42 spots/106 peripheral blood lymphocytes.

Original languageEnglish (US)
Pages (from-to)1127-1129
Number of pages3
JournalClinical and Diagnostic Laboratory Immunology
Volume12
Issue number9
DOIs
StatePublished - Sep 2005

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Clinical Biochemistry
  • Microbiology (medical)

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