Comparison of the Abbott m2000 HIV-1 real-time and Roche AMPLICOR monitor v1.5 HIV-1 assays on plasma specimens from Rakai, Uganda

I. Ssebugenyi, A. Kizza, B. Mpoza, G. Aluma, I. Boaz, K. Newell, O. Laeyendecker, J. P. Shott, D. Serwadda, S. J. Reynolds

Research output: Contribution to journalArticlepeer-review

Abstract

Summary: The need for viral load (VL) monitoring of HIV patients receiving antiretroviral therapy (ART) in resource-limited settings (RLS) has become apparent with studies showing the limitations of immunological monitoring. We compared the Abbott m2000 Real-Time (Abbott) HIV-1 assay with the Roche AMPLICOR Monitor v1.5 (Roche) HIV-1 assay over a range of VL concentrations. Three hundred and eleven plasma samples were tested, including 164 samples from patients on ART ≥ six months and 147 from ART-nai{dotless}̈ve patients. The Roche assay detected ≥400 copies/mL in 158 (50.8%) samples. Of these, Abbott produced 145 (91.8%) detectable results ≥400 copies/mL; 13 (8.2%) samples produced discrepant results. Concordance between the assays for detecting HIV-1 RNA ≥400 copies/mL was 95.8% (298/311). The sensitivity, specificity, positive predictive value and negative predictive value of Abbott to detect HIV-1 RNA ≥400 copies/mL were 91.8%, 100%, 100% and 92.2%, respectively. For the 151 samples with HIV-1 RNA ≥400 copies/mL for both assays, a good linear correlation was found (r = 0.81, P<0.0001; mean difference, 0.05). The limits of agreement were -0.97 and 1.07 log10 copies/mL (mean±2 SD). The Abbott assay performed well in our setting, offering an alternative methodology for HIV-1 VL for laboratories with realtime polymerase chain reaction (PCR) capacity.

Original languageEnglish (US)
Pages (from-to)373-375
Number of pages3
JournalInternational Journal of STD and AIDS
Volume22
Issue number7
DOIs
StatePublished - Jul 2011

Keywords

  • Antiretroviral treatment
  • HIV-1
  • Monitoring
  • Realtime polymerase chain reaction
  • Resource-limited
  • Viral load

ASJC Scopus subject areas

  • Dermatology
  • Public Health, Environmental and Occupational Health
  • Pharmacology (medical)
  • Infectious Diseases

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