Comparison of serum vitamin A values obtained by three different analytical methods

A. A. Rider, A. Sommer

Research output: Contribution to journalArticlepeer-review

Abstract

A comparison of biochemical methods used in field studies was conducted by determining vitamin A levels in sera from 12 biliary cirrhosis patients and 6 cystic fibrosis patients. High Performance Liquid Chromatography (HPLC), Trifluoroacetic Acid Colorimetry (TFA), and spectrophotometry with ultraviolet inactivation (UV) were tested. There was no significant difference in the mean values obtained by the three methods for each group of patients but some individual values showed wide differences. Correlation coefficients for values obtained by HPLC vs. TFA and UV were 0.92 and 0.93, respectively. Correlation coefficients vs. total retinol binding protein were 0.93, 0.87, and 0.82 for HPLC, TFA, and UV, respectively. HPLC showed the highest sensitivity and least interference from other serum components. Interference by carotenoids and other lipids was high in the TFA method. Choice of method to be used in a specific field situation depends largely on the sophistication of the laboratory equipment available.

Original languageEnglish (US)
Pages (from-to)891-897
Number of pages7
JournalNutrition Reports International
Volume28
Issue number4
StatePublished - Jan 1 1983

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science
  • Biochemistry
  • Endocrinology

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