Comparison of sensitivities and specificities of latex agglutination and an enzyme-linked immunosorbent assay for detection of antibodies to the human immunodeficiency virus in African sera

H. L. Francis, M. Kabeya, N. Kafuama, C. Riggins, R. Colebunders, R. Ryder, J. Curran, L. Izaley, Thomas C Quinn

Research output: Contribution to journalArticle

Abstract

The sensitivities, specificities, and positive and negative predictive values of the Cambridge BioScience Corp. (Worcester, Mass.) human immunodeficiency virus latex agglutination assay were compared by using three different blood preparations. By using the manufacturer's standard test method with diluted sera, the sensitivity of latex agglutination was 100%, the specificity was 99.58%, and the positive and negative predictive values were 99.26 and 100%, respectively. Use of diluted whole blood or undiluted whole blood did not significantly affect the sensitivity (mean, 99.72%), specificity (mean, 99.47%), positive predictive value (mean, 99.07%), or negative predictive value (mean, 99.89%). The latex agglutination assay is a simple, rapid assay for the detection of human immunodeficiency virus that would be useful in the Third World countries or other areas where enzyme-linked immunosorbent assays are not available or cannot be used.

Original languageEnglish (US)
Pages (from-to)2462-2464
Number of pages3
JournalJournal of Clinical Microbiology
Volume26
Issue number11
StatePublished - 1988
Externally publishedYes

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Agglutination
Latex
Enzyme-Linked Immunosorbent Assay
HIV
Sensitivity and Specificity
Antibodies
Serum
Developing Countries

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Comparison of sensitivities and specificities of latex agglutination and an enzyme-linked immunosorbent assay for detection of antibodies to the human immunodeficiency virus in African sera. / Francis, H. L.; Kabeya, M.; Kafuama, N.; Riggins, C.; Colebunders, R.; Ryder, R.; Curran, J.; Izaley, L.; Quinn, Thomas C.

In: Journal of Clinical Microbiology, Vol. 26, No. 11, 1988, p. 2462-2464.

Research output: Contribution to journalArticle

Francis, H. L. ; Kabeya, M. ; Kafuama, N. ; Riggins, C. ; Colebunders, R. ; Ryder, R. ; Curran, J. ; Izaley, L. ; Quinn, Thomas C. / Comparison of sensitivities and specificities of latex agglutination and an enzyme-linked immunosorbent assay for detection of antibodies to the human immunodeficiency virus in African sera. In: Journal of Clinical Microbiology. 1988 ; Vol. 26, No. 11. pp. 2462-2464.
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AU - Riggins, C.

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AU - Ryder, R.

AU - Curran, J.

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