Comparison of results obtained with amplicor HIV-1 DNA PCR test version 1.5 using 100 versus 500 microliters of whole blood

Estelle Piwowar-Manning, Lebah Lugalia, Bosco Kafufu, J. Brooks Jackson

Research output: Contribution to journalArticlepeer-review

Abstract

The Amplicor HIV-1 DNA PCR assay (Roche Diagnostics, Branchburg, NJ) requires 500 μl of whole blood for a diagnosis of human immunodeficiency virus type 1 (HIV-1) infection, and this amount is often difficult to obtain from infants. A comparison was performed using 100 and 500 μl of whole blood from infants less than 18 months of age. The concordance rate for HIV DNA PCR-negative and -positive samples was 100% for the two different volumes.

Original languageEnglish (US)
Pages (from-to)1104-1105
Number of pages2
JournalJournal of Clinical Microbiology
Volume46
Issue number3
DOIs
StatePublished - Mar 2008

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

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