Comparison of quantitative and semiquantitative enzyme-linked immunosorbent assays for immunoglobulin G against Chlamydophila pneumoniae to a microimmunofluorescence test for use with patients with respiratory tract infections

Corinna Hermann, Katja Gueinzius, Albrecht Oehme, Sonja Von Aulock, Eberhard Straube, Thomas Hartung

Research output: Contribution to journalArticle

Abstract

We previously reported a high degree of variation in the sensitivities of serodiagnostic kits for the detection of Chlamydophila pneumoniae in sera from healthy donors. Since a low predictive value of a test can impair its diagnostic value, we have extended our studies to samples from patients with pneumonia. We focused on the most promising enzyme-linked immunosorbent assays (ELISAs) (SeroCP and SeroCP Quant; Savyon) identified in our previous study and included a new ELISA (sELISA; Medac). The agreement between all ELISAs for immunoglobulin G (IgG) and a reference microimmunofluorescence (MIF) test for IgG (SeroFIA; Savyon) was ≥ 90% for a collective of 80 patients. The positive predictive values were all ≥ 93%. The negative predictive values ranged from 68 to 83%. False-negative results were obtained only for samples that had low titers in the MIF test. The correlation of the IgG antibody titers determined by the MIF and SeroCP Quant tests was high (rsp = 0.9). Since the semiquantitative SeroCP and quantitative SeroCP Quant ELISAs achieved the highest sensitivities, they were evaluated further by using a second batch of sera from 50 patients with predominantly medium and low antibody titers in the MIF test and a control collection of sera from 80 children with negative MIF results. Again, the tests showed a high concordance with the MIF results (96%), and the antibody titers in the SeroCP Quant and MIF tests correlated well (rsp = 0.8). The specificities determined with the negative sera were ≥ 99% for the SeroCP Quant test and 86% for the SeroCP test. These results show that ELISAs that are fast and objective deliver seroprevalence results, sensitivities, and specificities that are very similar to those of the MIF test.

Original languageEnglish (US)
Pages (from-to)2476-2479
Number of pages4
JournalJournal of Clinical Microbiology
Volume42
Issue number6
DOIs
StatePublished - Jun 2004
Externally publishedYes

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Chlamydophila pneumoniae
Respiratory Tract Infections
Immunoglobulin G
Enzyme-Linked Immunosorbent Assay
Serum
Antibodies
Predictive Value of Tests
Seroepidemiologic Studies
Pneumonia
Tissue Donors
Sensitivity and Specificity

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Comparison of quantitative and semiquantitative enzyme-linked immunosorbent assays for immunoglobulin G against Chlamydophila pneumoniae to a microimmunofluorescence test for use with patients with respiratory tract infections. / Hermann, Corinna; Gueinzius, Katja; Oehme, Albrecht; Von Aulock, Sonja; Straube, Eberhard; Hartung, Thomas.

In: Journal of Clinical Microbiology, Vol. 42, No. 6, 06.2004, p. 2476-2479.

Research output: Contribution to journalArticle

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abstract = "We previously reported a high degree of variation in the sensitivities of serodiagnostic kits for the detection of Chlamydophila pneumoniae in sera from healthy donors. Since a low predictive value of a test can impair its diagnostic value, we have extended our studies to samples from patients with pneumonia. We focused on the most promising enzyme-linked immunosorbent assays (ELISAs) (SeroCP and SeroCP Quant; Savyon) identified in our previous study and included a new ELISA (sELISA; Medac). The agreement between all ELISAs for immunoglobulin G (IgG) and a reference microimmunofluorescence (MIF) test for IgG (SeroFIA; Savyon) was ≥ 90{\%} for a collective of 80 patients. The positive predictive values were all ≥ 93{\%}. The negative predictive values ranged from 68 to 83{\%}. False-negative results were obtained only for samples that had low titers in the MIF test. The correlation of the IgG antibody titers determined by the MIF and SeroCP Quant tests was high (rsp = 0.9). Since the semiquantitative SeroCP and quantitative SeroCP Quant ELISAs achieved the highest sensitivities, they were evaluated further by using a second batch of sera from 50 patients with predominantly medium and low antibody titers in the MIF test and a control collection of sera from 80 children with negative MIF results. Again, the tests showed a high concordance with the MIF results (96{\%}), and the antibody titers in the SeroCP Quant and MIF tests correlated well (rsp = 0.8). The specificities determined with the negative sera were ≥ 99{\%} for the SeroCP Quant test and 86{\%} for the SeroCP test. These results show that ELISAs that are fast and objective deliver seroprevalence results, sensitivities, and specificities that are very similar to those of the MIF test.",
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