Comparison of PSA-specific CD8+ CTL responses and antitumor immunity generated by plasmid DNA vaccines encoding PSA-HSP chimeric proteins

Maxim Pavlenko, Anna Karin Roos, Christoph Leder, Lars Olof Hansson, Rolf Kiessling, Elena Levitskaya, Pavel Pisa

Research output: Contribution to journalArticlepeer-review

Abstract

The ability of heat shock proteins (HSPs) to increase the potency of protein- and DNA-based vaccines has been previously reported. We have constructed several plasmid-based vectors encoding chimeric proteins containing prostate-specific antigen (PSA) fused to Mycobacterium tuberculosis hsp70, M. bovis hsp65, Escherichia coli DnaK (hsp70), or human hsp70. Immunizing mice with these plasmids induced CD8+ cytotoxic T lymphocytes (CTLs) specific to human PSA and protected mice from a subsequent subcutaneous challenge with PSA-expressing tumors. We did not observe a significant difference either in the levels of PSA-specific CTLs or in protection against tumor challenge in mice immunized with plasmids expressing PSA-HSP chimeric proteins, as compared to mice receiving a conventional PSA-expressing DNA plasmid. Our data indicate that using HSPs as fusion partners for tumor-specific antigens does not always result in the enhancement of antigen-specific CTL responses when applied in the form of DNA vaccines.

Original languageEnglish (US)
Pages (from-to)1085-1092
Number of pages8
JournalCancer Immunology Immunotherapy
Volume53
Issue number12
DOIs
StatePublished - Dec 2004
Externally publishedYes

Keywords

  • CTL
  • DNA vaccine
  • Heat shock protein
  • Prostate-specific antigen

ASJC Scopus subject areas

  • Cancer Research
  • Immunology
  • Oncology

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