Steady state mRNA levels for transforming growth factor β, platelet-derived growth factor (PDGF) A-chain, and PDGF B-chain were measured in normal human mesothelial cells, SV40 large T-antigen expressing human mesothelial cells, and human mesothelioma cell lines. The mRNA expression level for transforming growth factor β was similar in all three types of culture while normal human mesothelial cells secrete more transforming growth factor β than do mesothelioma cell lines or T-antigen expressing mesothelial cells. In contrast, both PDGF A-and B-chain mRNAs are expressed at higher levels in mesothelioma cell lines than in normal human mesothelial cells. PDGF-like mitogenic activity was readily detectable in medium conditioned by a mesothelioma cell line and was undetectable in conditioned medium from normal cells. These results suggest the hypothesis that PDGF may be an autocrine growth factor in mesothelioma.
|Original language||English (US)|
|Number of pages||5|
|State||Published - Dec 1987|
ASJC Scopus subject areas
- Cancer Research