Comparison of Production of Transforming Growth Factor-β and Platelet-derived Growth Factor by Normal Human Mesothelial Cells and Mesothelioma Cell Lines

Brenda I. Gerwin; John F. Lechner; Roger R. Reddel; Edward W. Gabrielson; Curtis C. Harris

Comparison of Production of Transforming Growth Factor-β and Platelet-derived Growth Factor by Normal Human Mesothelial Cells and Mesothelioma Cell Lines

Brenda I. Gerwin, John F. Lechner, Roger R. Reddel, Edward W. Gabrielson, Curtis C. Harris

Research output: Contribution to journal › Article › peer-review

Abstract

Steady state mRNA levels for transforming growth factor β, platelet-derived growth factor (PDGF) A-chain, and PDGF B-chain were measured in normal human mesothelial cells, SV40 large T-antigen expressing human mesothelial cells, and human mesothelioma cell lines. The mRNA expression level for transforming growth factor β was similar in all three types of culture while normal human mesothelial cells secrete more transforming growth factor β than do mesothelioma cell lines or T-antigen expressing mesothelial cells. In contrast, both PDGF A-and B-chain mRNAs are expressed at higher levels in mesothelioma cell lines than in normal human mesothelial cells. PDGF-like mitogenic activity was readily detectable in medium conditioned by a mesothelioma cell line and was undetectable in conditioned medium from normal cells. These results suggest the hypothesis that PDGF may be an autocrine growth factor in mesothelioma.

Original language	English (US)
Pages (from-to)	6180-6184
Number of pages	5
Journal	Cancer Research
Volume	47
Issue number	23
State	Published - Dec 1987
Externally published	Yes

ASJC Scopus subject areas

Oncology
Cancer Research

Cite this

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title = "Comparison of Production of Transforming Growth Factor-β and Platelet-derived Growth Factor by Normal Human Mesothelial Cells and Mesothelioma Cell Lines",

abstract = "Steady state mRNA levels for transforming growth factor β, platelet-derived growth factor (PDGF) A-chain, and PDGF B-chain were measured in normal human mesothelial cells, SV40 large T-antigen expressing human mesothelial cells, and human mesothelioma cell lines. The mRNA expression level for transforming growth factor β was similar in all three types of culture while normal human mesothelial cells secrete more transforming growth factor β than do mesothelioma cell lines or T-antigen expressing mesothelial cells. In contrast, both PDGF A-and B-chain mRNAs are expressed at higher levels in mesothelioma cell lines than in normal human mesothelial cells. PDGF-like mitogenic activity was readily detectable in medium conditioned by a mesothelioma cell line and was undetectable in conditioned medium from normal cells. These results suggest the hypothesis that PDGF may be an autocrine growth factor in mesothelioma.",

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AU - Lechner, John F.

AU - Reddel, Roger R.

AU - Gabrielson, Edward W.

AU - Harris, Curtis C.

PY - 1987/12

Y1 - 1987/12

N2 - Steady state mRNA levels for transforming growth factor β, platelet-derived growth factor (PDGF) A-chain, and PDGF B-chain were measured in normal human mesothelial cells, SV40 large T-antigen expressing human mesothelial cells, and human mesothelioma cell lines. The mRNA expression level for transforming growth factor β was similar in all three types of culture while normal human mesothelial cells secrete more transforming growth factor β than do mesothelioma cell lines or T-antigen expressing mesothelial cells. In contrast, both PDGF A-and B-chain mRNAs are expressed at higher levels in mesothelioma cell lines than in normal human mesothelial cells. PDGF-like mitogenic activity was readily detectable in medium conditioned by a mesothelioma cell line and was undetectable in conditioned medium from normal cells. These results suggest the hypothesis that PDGF may be an autocrine growth factor in mesothelioma.

AB - Steady state mRNA levels for transforming growth factor β, platelet-derived growth factor (PDGF) A-chain, and PDGF B-chain were measured in normal human mesothelial cells, SV40 large T-antigen expressing human mesothelial cells, and human mesothelioma cell lines. The mRNA expression level for transforming growth factor β was similar in all three types of culture while normal human mesothelial cells secrete more transforming growth factor β than do mesothelioma cell lines or T-antigen expressing mesothelial cells. In contrast, both PDGF A-and B-chain mRNAs are expressed at higher levels in mesothelioma cell lines than in normal human mesothelial cells. PDGF-like mitogenic activity was readily detectable in medium conditioned by a mesothelioma cell line and was undetectable in conditioned medium from normal cells. These results suggest the hypothesis that PDGF may be an autocrine growth factor in mesothelioma.

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Comparison of Production of Transforming Growth Factor-β and Platelet-derived Growth Factor by Normal Human Mesothelial Cells and Mesothelioma Cell Lines

Abstract

ASJC Scopus subject areas

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