Comparison of oxidant activities and the activation of benzo(a)pyrene-7,8-dihydrodiol by polymorphonuclear leucocytes from human, rat and mouse

M. A. Trush; L. E. Twerdok; R. L. Esterline

doi:10.3109/00498259009046908

Comparison of oxidant activities and the activation of benzo(a)pyrene-7,8-dihydrodiol by polymorphonuclear leucocytes from human, rat and mouse

M. A. Trush, L. E. Twerdok, R. L. Esterline

Bloomberg School of Public Health

Research output: Contribution to journal › Article › peer-review

Abstract

1. Polymorphonuclear leucocytes (PMNs) from human, mouse and rat were compared for their ability to activate benzo(a)pyrene-7,8-diol to a DNA-binding intermediate and a chemiluminescent dioxetane derivative. 2. Human PMNs activated benzo(a)pyrene-7,8-diol to the greatest extent, which correlated with their rate of superoxide anion generation and myeloperoxidase activity relative to these activities by PMNs from mice and rats. 3. Benzo(a)pyrene-7,8-diol chemiluminescence elicited by PMNs from all three species was significantly inhibited by azide, a myeloperoxidase inhibitor. On the other hand, superoxide dismutase (SOD) was very effective in inhibiting benzo(a)pyrene-7,8-diol chemiluminescence with mouse PMNs but not human or rat PMNs. Mouse PMNs exhibited a high rate of superoxide generation but low myeloperoxidase activity, whereas rat PMNs exhibited a low rate of superoxide generation but high myeloperoxidase activity.

Original language	English (US)
Pages (from-to)	925-932
Number of pages	8
Journal	Xenobiotica
Volume	20
Issue number	9
DOIs	https://doi.org/10.3109/00498259009046908
State	Published - Jan 1 1990

ASJC Scopus subject areas

Biochemistry
Toxicology
Pharmacology
Health, Toxicology and Mutagenesis

Access to Document

10.3109/00498259009046908

Fingerprint Dive into the research topics of 'Comparison of oxidant activities and the activation of benzo(a)pyrene-7,8-dihydrodiol by polymorphonuclear leucocytes from human, rat and mouse'. Together they form a unique fingerprint.

Cite this

@article{7ae615d29f5e465db7ce5c2bf4dc7c84,

title = "Comparison of oxidant activities and the activation of benzo(a)pyrene-7,8-dihydrodiol by polymorphonuclear leucocytes from human, rat and mouse",

abstract = "1. Polymorphonuclear leucocytes (PMNs) from human, mouse and rat were compared for their ability to activate benzo(a)pyrene-7,8-diol to a DNA-binding intermediate and a chemiluminescent dioxetane derivative. 2. Human PMNs activated benzo(a)pyrene-7,8-diol to the greatest extent, which correlated with their rate of superoxide anion generation and myeloperoxidase activity relative to these activities by PMNs from mice and rats. 3. Benzo(a)pyrene-7,8-diol chemiluminescence elicited by PMNs from all three species was significantly inhibited by azide, a myeloperoxidase inhibitor. On the other hand, superoxide dismutase (SOD) was very effective in inhibiting benzo(a)pyrene-7,8-diol chemiluminescence with mouse PMNs but not human or rat PMNs. Mouse PMNs exhibited a high rate of superoxide generation but low myeloperoxidase activity, whereas rat PMNs exhibited a low rate of superoxide generation but high myeloperoxidase activity.",

author = "Trush, {M. A.} and Twerdok, {L. E.} and Esterline, {R. L.}",

year = "1990",

month = jan,

day = "1",

doi = "10.3109/00498259009046908",

language = "English (US)",

volume = "20",

pages = "925--932",

journal = "Xenobiotica",

issn = "0049-8254",

publisher = "Informa Healthcare",

number = "9",

}

TY - JOUR

T1 - Comparison of oxidant activities and the activation of benzo(a)pyrene-7,8-dihydrodiol by polymorphonuclear leucocytes from human, rat and mouse

AU - Trush, M. A.

AU - Twerdok, L. E.

AU - Esterline, R. L.

PY - 1990/1/1

Y1 - 1990/1/1

N2 - 1. Polymorphonuclear leucocytes (PMNs) from human, mouse and rat were compared for their ability to activate benzo(a)pyrene-7,8-diol to a DNA-binding intermediate and a chemiluminescent dioxetane derivative. 2. Human PMNs activated benzo(a)pyrene-7,8-diol to the greatest extent, which correlated with their rate of superoxide anion generation and myeloperoxidase activity relative to these activities by PMNs from mice and rats. 3. Benzo(a)pyrene-7,8-diol chemiluminescence elicited by PMNs from all three species was significantly inhibited by azide, a myeloperoxidase inhibitor. On the other hand, superoxide dismutase (SOD) was very effective in inhibiting benzo(a)pyrene-7,8-diol chemiluminescence with mouse PMNs but not human or rat PMNs. Mouse PMNs exhibited a high rate of superoxide generation but low myeloperoxidase activity, whereas rat PMNs exhibited a low rate of superoxide generation but high myeloperoxidase activity.

AB - 1. Polymorphonuclear leucocytes (PMNs) from human, mouse and rat were compared for their ability to activate benzo(a)pyrene-7,8-diol to a DNA-binding intermediate and a chemiluminescent dioxetane derivative. 2. Human PMNs activated benzo(a)pyrene-7,8-diol to the greatest extent, which correlated with their rate of superoxide anion generation and myeloperoxidase activity relative to these activities by PMNs from mice and rats. 3. Benzo(a)pyrene-7,8-diol chemiluminescence elicited by PMNs from all three species was significantly inhibited by azide, a myeloperoxidase inhibitor. On the other hand, superoxide dismutase (SOD) was very effective in inhibiting benzo(a)pyrene-7,8-diol chemiluminescence with mouse PMNs but not human or rat PMNs. Mouse PMNs exhibited a high rate of superoxide generation but low myeloperoxidase activity, whereas rat PMNs exhibited a low rate of superoxide generation but high myeloperoxidase activity.

UR - http://www.scopus.com/inward/record.url?scp=0025117949&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025117949&partnerID=8YFLogxK

U2 - 10.3109/00498259009046908

DO - 10.3109/00498259009046908

M3 - Article

C2 - 2173286

AN - SCOPUS:0025117949

VL - 20

SP - 925

EP - 932

JO - Xenobiotica

JF - Xenobiotica

SN - 0049-8254

IS - 9

ER -