TY - JOUR
T1 - Comparison of oxidant activities and the activation of benzo(a)pyrene-7,8-dihydrodiol by polymorphonuclear leucocytes from human, rat and mouse
AU - Trush, M. A.
AU - Twerdok, L. E.
AU - Esterline, R. L.
N1 - Funding Information:
This research was supported by ES 03760, ES 07141 ) American Cancer Society SIG-3 and Johns Hopkins CAAT.
PY - 1990
Y1 - 1990
N2 - 1. Polymorphonuclear leucocytes (PMNs) from human, mouse and rat were compared for their ability to activate benzo(a)pyrene-7,8-diol to a DNA-binding intermediate and a chemiluminescent dioxetane derivative. 2. Human PMNs activated benzo(a)pyrene-7,8-diol to the greatest extent, which correlated with their rate of superoxide anion generation and myeloperoxidase activity relative to these activities by PMNs from mice and rats. 3. Benzo(a)pyrene-7,8-diol chemiluminescence elicited by PMNs from all three species was significantly inhibited by azide, a myeloperoxidase inhibitor. On the other hand, superoxide dismutase (SOD) was very effective in inhibiting benzo(a)pyrene-7,8-diol chemiluminescence with mouse PMNs but not human or rat PMNs. Mouse PMNs exhibited a high rate of superoxide generation but low myeloperoxidase activity, whereas rat PMNs exhibited a low rate of superoxide generation but high myeloperoxidase activity.
AB - 1. Polymorphonuclear leucocytes (PMNs) from human, mouse and rat were compared for their ability to activate benzo(a)pyrene-7,8-diol to a DNA-binding intermediate and a chemiluminescent dioxetane derivative. 2. Human PMNs activated benzo(a)pyrene-7,8-diol to the greatest extent, which correlated with their rate of superoxide anion generation and myeloperoxidase activity relative to these activities by PMNs from mice and rats. 3. Benzo(a)pyrene-7,8-diol chemiluminescence elicited by PMNs from all three species was significantly inhibited by azide, a myeloperoxidase inhibitor. On the other hand, superoxide dismutase (SOD) was very effective in inhibiting benzo(a)pyrene-7,8-diol chemiluminescence with mouse PMNs but not human or rat PMNs. Mouse PMNs exhibited a high rate of superoxide generation but low myeloperoxidase activity, whereas rat PMNs exhibited a low rate of superoxide generation but high myeloperoxidase activity.
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U2 - 10.3109/00498259009046908
DO - 10.3109/00498259009046908
M3 - Article
C2 - 2173286
AN - SCOPUS:0025117949
VL - 20
SP - 925
EP - 932
JO - Xenobiotica
JF - Xenobiotica
SN - 0049-8254
IS - 9
ER -