Abstract
1. Polymorphonuclear leucocytes (PMNs) from human, mouse and rat were compared for their ability to activate benzo(a)pyrene-7,8-diol to a DNA-binding intermediate and a chemiluminescent dioxetane derivative. 2. Human PMNs activated benzo(a)pyrene-7,8-diol to the greatest extent, which correlated with their rate of superoxide anion generation and myeloperoxidase activity relative to these activities by PMNs from mice and rats. 3. Benzo(a)pyrene-7,8-diol chemiluminescence elicited by PMNs from all three species was significantly inhibited by azide, a myeloperoxidase inhibitor. On the other hand, superoxide dismutase (SOD) was very effective in inhibiting benzo(a)pyrene-7,8-diol chemiluminescence with mouse PMNs but not human or rat PMNs. Mouse PMNs exhibited a high rate of superoxide generation but low myeloperoxidase activity, whereas rat PMNs exhibited a low rate of superoxide generation but high myeloperoxidase activity.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 925-932 |
| Number of pages | 8 |
| Journal | Xenobiotica |
| Volume | 20 |
| Issue number | 9 |
| DOIs | |
| State | Published - 1990 |
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ASJC Scopus subject areas
- Pharmacology
- Toxicology
- Biochemistry
- Health, Toxicology and Mutagenesis
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Comparison of oxidant activities and the activation of benzo(a)pyrene-7,8-dihydrodiol by polymorphonuclear leucocytes from human, rat and mouse. / Trush, M. A.; Twerdok, L. E.; Esterline, R. L.
In: Xenobiotica, Vol. 20, No. 9, 1990, p. 925-932.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comparison of oxidant activities and the activation of benzo(a)pyrene-7,8-dihydrodiol by polymorphonuclear leucocytes from human, rat and mouse
AU - Trush, M. A.
AU - Twerdok, L. E.
AU - Esterline, R. L.
PY - 1990
Y1 - 1990
N2 - 1. Polymorphonuclear leucocytes (PMNs) from human, mouse and rat were compared for their ability to activate benzo(a)pyrene-7,8-diol to a DNA-binding intermediate and a chemiluminescent dioxetane derivative. 2. Human PMNs activated benzo(a)pyrene-7,8-diol to the greatest extent, which correlated with their rate of superoxide anion generation and myeloperoxidase activity relative to these activities by PMNs from mice and rats. 3. Benzo(a)pyrene-7,8-diol chemiluminescence elicited by PMNs from all three species was significantly inhibited by azide, a myeloperoxidase inhibitor. On the other hand, superoxide dismutase (SOD) was very effective in inhibiting benzo(a)pyrene-7,8-diol chemiluminescence with mouse PMNs but not human or rat PMNs. Mouse PMNs exhibited a high rate of superoxide generation but low myeloperoxidase activity, whereas rat PMNs exhibited a low rate of superoxide generation but high myeloperoxidase activity.
AB - 1. Polymorphonuclear leucocytes (PMNs) from human, mouse and rat were compared for their ability to activate benzo(a)pyrene-7,8-diol to a DNA-binding intermediate and a chemiluminescent dioxetane derivative. 2. Human PMNs activated benzo(a)pyrene-7,8-diol to the greatest extent, which correlated with their rate of superoxide anion generation and myeloperoxidase activity relative to these activities by PMNs from mice and rats. 3. Benzo(a)pyrene-7,8-diol chemiluminescence elicited by PMNs from all three species was significantly inhibited by azide, a myeloperoxidase inhibitor. On the other hand, superoxide dismutase (SOD) was very effective in inhibiting benzo(a)pyrene-7,8-diol chemiluminescence with mouse PMNs but not human or rat PMNs. Mouse PMNs exhibited a high rate of superoxide generation but low myeloperoxidase activity, whereas rat PMNs exhibited a low rate of superoxide generation but high myeloperoxidase activity.
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UR - http://www.scopus.com/inward/citedby.url?scp=0025117949&partnerID=8YFLogxK
U2 - 10.3109/00498259009046908
DO - 10.3109/00498259009046908
M3 - Article
C2 - 2173286
AN - SCOPUS:0025117949
VL - 20
SP - 925
EP - 932
JO - Xenobiotica
JF - Xenobiotica
SN - 0049-8254
IS - 9
ER -