Comparison of oxidant activities and the activation of benzo(a)pyrene-7,8-dihydrodiol by polymorphonuclear leucocytes from human, rat and mouse

M. A. Trush, L. E. Twerdok, R. L. Esterline

Research output: Contribution to journalArticlepeer-review

Abstract

1. Polymorphonuclear leucocytes (PMNs) from human, mouse and rat were compared for their ability to activate benzo(a)pyrene-7,8-diol to a DNA-binding intermediate and a chemiluminescent dioxetane derivative. 2. Human PMNs activated benzo(a)pyrene-7,8-diol to the greatest extent, which correlated with their rate of superoxide anion generation and myeloperoxidase activity relative to these activities by PMNs from mice and rats. 3. Benzo(a)pyrene-7,8-diol chemiluminescence elicited by PMNs from all three species was significantly inhibited by azide, a myeloperoxidase inhibitor. On the other hand, superoxide dismutase (SOD) was very effective in inhibiting benzo(a)pyrene-7,8-diol chemiluminescence with mouse PMNs but not human or rat PMNs. Mouse PMNs exhibited a high rate of superoxide generation but low myeloperoxidase activity, whereas rat PMNs exhibited a low rate of superoxide generation but high myeloperoxidase activity.

Original languageEnglish (US)
Pages (from-to)925-932
Number of pages8
JournalXenobiotica
Volume20
Issue number9
DOIs
StatePublished - Jan 1 1990

ASJC Scopus subject areas

  • Biochemistry
  • Toxicology
  • Pharmacology
  • Health, Toxicology and Mutagenesis

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