Comparison of nuclear shape in aspirated and histologic specimens of prostatic carcinoma

C. P. Tardif, A. W. Partin, B. Qaqish, J. P. Epstein, J. L. Mohler

Research output: Contribution to journalArticlepeer-review

Abstract

Nuclear shape analysis of histologic section of operative specimens has separated patients with clinically localized prostatic carcinoma with good and poor prognoses. In order to become more useful clinically, nuclear shape should be evaluable preoperatively but is distorted by core biopsy. We used nuclear morphometry to compare histologic and cytologic specimens obtained from 20 patients who underwent radical prostatectomy for clinically localized prostatic carcinoma. Neoblastic nuclei from cytologic aspirates prepared prepared by the Papanicolaou and Diff- Quik techniques and standard histologic sections stained with hematoxylin and eosin were digitized. Air-dried nuclei of the Diff-Quik preparation had a nuclear area of 104.2μm2±28.2 SD. These nuclei were nearly twice as large (paired t test, P<.001) as in the Papanicolaou (55.6±13.0 μm2) and histologic (55.8±12.7 μm2) preparations. However, nuclear shape was not affected. Nuclear roundness factor (deviation from circularity) and boundary curvature were similar (paired t tests, P>.05). All nuclear shape descriptors of cytologic smears fell within intraobserver variation of measurements in histologic section. Nuclear shape is not altered in Papanicolaou- and Diff-Quik-stained cytologic specimens and should be tested for preoperative assessment of outcome in clinically localized prostatic carcinoma.

Original languageEnglish (US)
Pages (from-to)474-482
Number of pages9
JournalAnalytical and Quantitative Cytology and Histology
Volume14
Issue number6
StatePublished - Dec 1 1992
Externally publishedYes

ASJC Scopus subject areas

  • Anatomy
  • Histology

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