Comparison of new and traditional methods for detection of senile plaques in Alzheimer's disease

L. S. Raskin, M. D. Applegate, D. L. Price, Juan C Troncoso, J. C. Hedreen

Research output: Contribution to journalArticle

Abstract

The pathologic diagnosis of Alzheimer's disease (AD) rests upon the identification of senile plaques and neurofibrillary tangles (NFT) in brain tissue, Methods for staining these structures vary in their sensitivity, Six different silver stains and immunocytochemistry for the β-amyloid (Aβ) peptide were compared for sensitivity in staining of plaques and tangles. For staining of plaques, the silver stains fell into two groups: one group stained primarily large, distorted neurites in classic plaques, and the other stained primarily fine, filamentous elements in diffuse plaques and at the periphery of classic plaques. Our recently developed 'quick silver' method demonstrated the highest number of plaques. Sensitivity of NFT staining also varied considerably. The quick silver and Yamamoto-Hirano methods were best for staining both plaques and NFT.

Original languageEnglish (US)
Pages (from-to)125-131
Number of pages7
JournalJournal of Geriatric Psychiatry and Neurology
Volume8
Issue number2
StatePublished - 1995

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Amyloid Plaques
Neurofibrillary Tangles
Alzheimer Disease
Silver
Staining and Labeling
Coloring Agents
Silver Staining
Neurites
Amyloid
Immunohistochemistry
Peptides
Brain

ASJC Scopus subject areas

  • Geriatrics and Gerontology
  • Neuroscience(all)
  • Neuropsychology and Physiological Psychology

Cite this

Comparison of new and traditional methods for detection of senile plaques in Alzheimer's disease. / Raskin, L. S.; Applegate, M. D.; Price, D. L.; Troncoso, Juan C; Hedreen, J. C.

In: Journal of Geriatric Psychiatry and Neurology, Vol. 8, No. 2, 1995, p. 125-131.

Research output: Contribution to journalArticle

Raskin, L. S. ; Applegate, M. D. ; Price, D. L. ; Troncoso, Juan C ; Hedreen, J. C. / Comparison of new and traditional methods for detection of senile plaques in Alzheimer's disease. In: Journal of Geriatric Psychiatry and Neurology. 1995 ; Vol. 8, No. 2. pp. 125-131.
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