Comparison of molecular and conventional methods for typing of enteroviral isolates

Darly Joseph Manayani, R. V. Shaji, G. John Fletcher, Thomas Cherian, N. Murali, N. Sathish, Tilak Solomon, Chandran Gnanamuthu, Gopalan Sridharan

Research output: Contribution to journalArticle

Abstract

Twenty-eight enteroviral isolates obtained from various clinical specimens were typed by Lim-Benyesh-Melnick (LBM) pool-based neutralization, PCR-restriction fragment length polymorphism (RFLP), and partial sequencing of the VP1 region of the enteroviral genome. Sequencing was found to be a good alternative to LBM typing, while PCR-RFLP was inadequate for identification of enteroviral isolates.

Original languageEnglish (US)
Pages (from-to)1069-1070
Number of pages2
JournalJournal of Clinical Microbiology
Volume40
Issue number3
DOIs
StatePublished - Mar 20 2002
Externally publishedYes

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Restriction Fragment Length Polymorphisms
Polymerase Chain Reaction
Genome

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Manayani, D. J., Shaji, R. V., Fletcher, G. J., Cherian, T., Murali, N., Sathish, N., ... Sridharan, G. (2002). Comparison of molecular and conventional methods for typing of enteroviral isolates. Journal of Clinical Microbiology, 40(3), 1069-1070. https://doi.org/10.1128/JCM.40.3.1069-1070.2002

Comparison of molecular and conventional methods for typing of enteroviral isolates. / Manayani, Darly Joseph; Shaji, R. V.; Fletcher, G. John; Cherian, Thomas; Murali, N.; Sathish, N.; Solomon, Tilak; Gnanamuthu, Chandran; Sridharan, Gopalan.

In: Journal of Clinical Microbiology, Vol. 40, No. 3, 20.03.2002, p. 1069-1070.

Research output: Contribution to journalArticle

Manayani, DJ, Shaji, RV, Fletcher, GJ, Cherian, T, Murali, N, Sathish, N, Solomon, T, Gnanamuthu, C & Sridharan, G 2002, 'Comparison of molecular and conventional methods for typing of enteroviral isolates', Journal of Clinical Microbiology, vol. 40, no. 3, pp. 1069-1070. https://doi.org/10.1128/JCM.40.3.1069-1070.2002
Manayani, Darly Joseph ; Shaji, R. V. ; Fletcher, G. John ; Cherian, Thomas ; Murali, N. ; Sathish, N. ; Solomon, Tilak ; Gnanamuthu, Chandran ; Sridharan, Gopalan. / Comparison of molecular and conventional methods for typing of enteroviral isolates. In: Journal of Clinical Microbiology. 2002 ; Vol. 40, No. 3. pp. 1069-1070.
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