HIV enfeksiyonunun moleküler tanisinda manuel ve otomatize (MagNa Pure) nükleik asit izolasyon yöntemlerinin karşilaştirilmasi

Translated title of the contribution: Comparison of manual and automated (MagNa Pure) nucleic acid isolation methods in molecular diagnosis of HIV infections

Alpaslan Alp, Dürdal Us, Gülşen Hasļik

Research output: Contribution to journalArticle

Abstract

Rapid quantitative molecular methods are very important for the diagnosis of human immunodeficiency virus (HIV) infections, assessment of prognosis and follow up. The purpose of this study was to compare and evaluate the performances of conventional manual extraction method and automated MagNA Pure system, for the nucleic acid isolation step which is the first and most important step in molecular diagnosis of HIV infections. Plasma samples of 35 patients in which anti-HIV antibodies were found as positive by microparticule enzyme immunoassay and confirmed by immunoblotting method, were included in the study. The nucleic acids obtained simultaneously by manual isolation kit (Cobas Amplicor, HIV-1 Monitor Test, version 1.5, Roche Diagnostics) and automated system (MagNA Pure LC Total Nucleic Acid Isolation Kit, Roche Diagnostics), were amplified and detected in Cobas Amplicor (Roche Diagnostics) instrument. Twenty three of 35 samples (65.7%) were found to be positive, and 9 (25.7%) were negative by both of the methods. The agreement between the methods were detected as 91.4%, for qualitative results. Viral RNA copies detected by manual and MagNA Pure isolation methods were found between 76.0-7.590.000 (mean: 487.143) and 113.0-20.300.0000 (mean: 2.174.097) copies/ml, respectively. When both of the overall and individual results were evaluated, the number of RNA copies obtained with automatized system, were found higher than the manual method (p

Original languageTurkish
Pages (from-to)77-83
Number of pages7
JournalMikrobiyoloji Bulteni
Volume38
Issue number1-2
StatePublished - Jan 2004
Externally publishedYes

Fingerprint

Virus Diseases
Nucleic Acids
HIV
Viral RNA
Immunoenzyme Techniques
Immunoblotting
HIV-1
RNA
Antibodies

Keywords

  • Cobas Amplicor
  • Human immunodeficiency virus (HIV)
  • Magna Pure
  • Nucleic acid isolation
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Infectious Diseases
  • Microbiology (medical)

Cite this

HIV enfeksiyonunun moleküler tanisinda manuel ve otomatize (MagNa Pure) nükleik asit izolasyon yöntemlerinin karşilaştirilmasi. / Alp, Alpaslan; Us, Dürdal; Hasļik, Gülşen.

In: Mikrobiyoloji Bulteni, Vol. 38, No. 1-2, 01.2004, p. 77-83.

Research output: Contribution to journalArticle

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